Kinetics of the anti-amebic antibody producing cells response in Peyer's patches and spleen after both local and systemic stimulation in Balb/c mice.

The kinetics of the anti-amebic antibody producing cell (APC) response was analyzed by a spot forming cell (SFC) assay, after a single dose of glutaraldehyde fixed amebas (GFA) by intragastric, rectal and intraperitoneal routes. It was found that mice elicited both local and systemic immune responses in Peyer's patches and spleen, by either local or systemic stimulation. The analysis of the isotype of the anti-amebic APC in mice given four times the GFA by the mentioned routes revealed that mice produced anti-amebic APC of the three major isotypes in both spleen and Peyer's patches.