Weeks K M, Cech T R
Department of Chemistry and Biochemistry, Howard Hughes Medical Institute, University of Colorado, Boulder 80309-0215, USA.
Science. 1996 Jan 19;271(5247):345-8. doi: 10.1126/science.271.5247.345.
CBP2 is an RNA tertiary structure binding protein required for efficient splicing of a yeast mitochondrial group I intron. CBP2 must wait for folding of the two RNA domains that make up the catalytic core before it can bind. In a subsequent step, association of the 5' domain of the RNA is stabilized by additional interactions with the protein. Thus, CBP2 functions primarily to capture otherwise transient RNA tertiary structures. This simple one-RNA, one-protein system has revealed how the kinetic pathway of RNA folding can direct the assembly of a specific ribonucleoprotein complex. There are parallels to steps in the formation of a much more complex ribonucleoprotein, the 30S ribosomal subunit.
CBP2是一种RNA三级结构结合蛋白，是酵母线粒体I组内含子有效剪接所必需的。CBP2必须等待构成催化核心的两个RNA结构域折叠完成后才能结合。在随后的步骤中，RNA的5'结构域与该蛋白的额外相互作用使其结合得以稳定。因此，CBP2的主要功能是捕获原本短暂存在的RNA三级结构。这个简单的单RNA、单蛋白系统揭示了RNA折叠的动力学途径如何指导特定核糖核蛋白复合物的组装。这与更为复杂的核糖核蛋白30S核糖体亚基形成过程中的步骤存在相似之处。
