van Susante J L, Buma P, van Osch G J, Versleyen D, van der Kraan P M, van der Berg W B, Homminga G N
Department of Orthopedics, University Hospital Nijmegen, The Netherlands.
Acta Orthop Scand. 1995 Dec;66(6):549-56. doi: 10.3109/17453679509002314.
In this in vitro study, we compared the potential of collagen and alginate gels as carriers for chondrocyte transplantation and we studied the influence of demineralized bone matrix (DBM) on chondrocytes in the gels. Chondrocytes were assessed for cell viability, phenotype (histology), proliferation rate and sulfate incorporation. Collagen gels showed a significant increase in cell numbers, but the chondrocytes dedifferentiated into fibroblast-like cells from day 6 onwards. In alginate gels, initial cell loss was found, but the cells maintained their typical chondrocyte phenotype. Although the total quantity of proteoglycans initially synthesized per cell in collagen gel was significantly higher, expressed per cell, the quantity in alginate gel eventually surpassed collagen. No effects of culturing chondrocytes in combination with DBM could be demonstrated on cell proliferation and sulfate incorporation. The collagen and alginate gels have different advantages as carriers for chondrocyte transplantation. The high proliferation rate of chondrocytes in collagen gel may be an advantage, but the preservation of the chondrocyte phenotype and the gradually increasing proteoglycan synthesis in alginate gel is a promising method for creating a hyaline cartilage implant in vitro.
在这项体外研究中，我们比较了胶原蛋白凝胶和海藻酸盐凝胶作为软骨细胞移植载体的潜力，并研究了脱矿骨基质（DBM）对凝胶中软骨细胞的影响。对软骨细胞进行了细胞活力、表型（组织学）、增殖率和硫酸盐掺入的评估。胶原蛋白凝胶中的细胞数量显著增加，但从第6天起软骨细胞开始去分化为成纤维细胞样细胞。在海藻酸盐凝胶中，最初发现有细胞损失，但细胞维持了其典型的软骨细胞表型。虽然最初每个细胞在胶原蛋白凝胶中合成的蛋白聚糖总量显著更高，但按每个细胞计算，海藻酸盐凝胶中的蛋白聚糖量最终超过了胶原蛋白凝胶。未证明将软骨细胞与DBM联合培养对细胞增殖和硫酸盐掺入有影响。胶原蛋白凝胶和海藻酸盐凝胶作为软骨细胞移植载体具有不同的优势。软骨细胞在胶原蛋白凝胶中的高增殖率可能是一个优势，但在海藻酸盐凝胶中保持软骨细胞表型以及蛋白聚糖合成逐渐增加是在体外创建透明软骨植入物的一种有前景的方法。
