Pardanaud L, Dieterlen-Lièvre F
Institut d'Embryologie Cellulaire et Moléculaire du CNRS et du Collège de France, Nogent-sur-Marne, France.
Anat Embryol (Berl). 1995 Oct;192(4):301-8. doi: 10.1007/BF00710099.
In a previous study of the hemangioblastic capacity of lateral plate mesoderm, we showed that the endoderm-associated splanchnopleural layer is capable of giving rise to both endothelial and hemopoietic cells while the ectoderm-associated somatopleural layer is not (Pardanaud and Dieterlen-Lièvre 1993a). In order to complete the inventory of territories able to produce these two cell lineages, we assayed the paraxial mesoderm, and report the results here. Quail somites or segmental plates were treated with mab QH1+complement in order to eliminate attached aortic endothelial cells, which cling to the ventral aspects of these structures. They were grafted in the limb bud or the coelom of chick host, since these sites promote the differentiation of endothelial and hemopoietic cells, respectively. Vascular development and hemopoietic cell emergence were analyzed using QH1 immunocytology. Segmental plate and somites both produced abundant endothelial cells. In addition, the segmental plate gave rise to small groups of hemopoietic cells when grafted in the coelom.
在先前一项关于侧板中胚层血管母细胞形成能力的研究中，我们发现与内胚层相关的脏壁层能够产生内皮细胞和造血细胞，而与外胚层相关的体壁层则不能（帕尔达诺和迪特尔伦-利夫尔，1993a）。为了完成能够产生这两种细胞谱系的区域清单，我们检测了轴旁中胚层，并在此报告结果。用单克隆抗体QH1加补体处理鹌鹑体节或节段板，以清除附着在这些结构腹侧的主动脉内皮细胞。将它们移植到鸡宿主的肢芽或体腔中，因为这些部位分别促进内皮细胞和造血细胞的分化。使用QH1免疫细胞化学分析血管发育和造血细胞的出现情况。节段板和体节都产生了大量内皮细胞。此外，节段板移植到体腔中时会产生少量造血细胞群。
