Pardanaud L, Dieterlen-Lièvre F
Institut d'Embryologie Cellulaire et Moléculaire du CNRS et du Collège de France, Nogent-sur-Marne.
Cell Adhes Commun. 1993 Sep;1(2):151-60. doi: 10.3109/15419069309095691.
In situ hybridization was used to detect the expression of the c-ets1 protooncogene during formation of the germ layers in the chick blastodisc. c-ets1 transcripts were present during the gastrulation process, i.e. when the mesodermal cells invaginated. The expression became down-regulated in lateral plate and the dorsal part of the somites while an intense signal was retained in the intermediate cell mass. When vasculogenesis started, c-ets1 transcripts labelled blood islands and endothelial cells. Before the mesoderm split, transcripts were present over the whole layer, more abundant however on its ventral side in contact with the endoderm. After the mesoderm split, silver grains became distributed asymmetrically: splanchnopleural mesoderm expressed c-ets1 messengers all over while expression in the somatopleural mesoderm was restricted to a few profiles corresponding to small endothelial cell groups. This asymmetrical distribution of c-ets1 transcripts is in agreement with our previous experimental findings establishing the different potentialities of the two mesodermal layers regarding hemopoiesis, vasculogenesis and angiogenesis processes.
原位杂交技术被用于检测鸡胚盘胚层形成过程中c-ets1原癌基因的表达。在原肠胚形成过程中，即中胚层细胞内陷时，可检测到c-ets1转录本。侧板和体节背部的表达下调，而中间细胞团中保留了强烈信号。血管发生开始时，c-ets1转录本标记血岛和内皮细胞。中胚层分裂前，转录本存在于整个中胚层，然而在与内胚层接触的腹侧更为丰富。中胚层分裂后，银颗粒分布不对称：脏壁中胚层在整个区域表达c-ets1信使，而体壁中胚层的表达局限于对应小内皮细胞群的少数细胞轮廓。c-ets1转录本的这种不对称分布与我们之前的实验结果一致，该结果确定了两个中胚层在造血、血管发生和血管生成过程方面的不同潜能。
