Chen L J, Hecht S S, Peterson L A
Division of Chemical Carcinogenesis, American Health Foundation, Valhalla, New York 10595, USA.
Chem Res Toxicol. 1995 Oct-Nov;8(7):903-6. doi: 10.1021/tx00049a001.
The hepatocarcinogen furan is believed to be activated to the reactive aldehyde, cis-2-butene-1,4-dial, by microsomal enzymes. The rat liver microsomal metabolism of furan was examined in the presence of NADPH and semicarbazide. HPLC analysis of incubation mixtures revealed the formation of a metabolite that coeluted with standards for the bis-semicarbazone adduct of cis-2-butene-1,4-dial. The formation of this compound required the presence of NADPH, semicarbazide, and microsomes. Preparative isolation and chemical characterization of this metabolite confirmed the structural assignment. These data provide evidence that the reactive aldehyde, cis-2-butene-1,4-dial, is a major metabolic product of furan.
肝癌致癌物呋喃被认为可通过微粒体酶激活为活性醛顺式-2-丁烯-1,4-二醛。在存在烟酰胺腺嘌呤二核苷酸磷酸（NADPH）和氨基脲的情况下，对大鼠肝脏微粒体中呋喃的代谢进行了研究。对孵育混合物的高效液相色谱（HPLC）分析显示形成了一种代谢物，其与顺式-2-丁烯-1,4-二醛双缩氨基脲加合物的标准品共洗脱。该化合物的形成需要NADPH、氨基脲和微粒体的存在。该代谢物的制备分离和化学表征证实了结构归属。这些数据提供了证据，表明活性醛顺式-2-丁烯-1,4-二醛是呋喃的主要代谢产物。
