González C R, Isibasi A, Ortiz-Navarrete V, Paniagua J, García J A, Ramirez A, Salvatierra B, Tapia R, Sepúlveda J, Gutiérrez G
Laboratorio de Inmunoquímica, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México, D.F., México.
Epidemiol Infect. 1995 Dec;115(3):535-43. doi: 10.1017/s0950268800058702.
The prevalence of antibodies against Entamoeba histolytica was studied in the Mexican population using an immunoenzyme assay in solid phase (ELISA) and semiautomatic equipment. The antigen was a mixture of membrane proteins obtained by Triton X-100 extraction from an axenic culture of Entamoeba histolytica HM1-IMSS. The method was standardized by comparing serum samples from amoebic liver abscess patients with healthy volunteers. From the 60,538 samples supplied by the National Seroepidemiology Survey, antibodies were found in 4.49% (4.32-4.65% at 95% confidence limit). More significant titres occurred in the central region of the country. The ratio female to male was 1.25:1. The population living in metropolitan areas had probably been infected at a younger age than those living in the country. Important differences were found in the seroprevalence obtained by ELISA compared with a study which used indirect haemagglutination (IHA) in the same sample frame.
采用固相免疫酶测定法（ELISA）和半自动设备，对墨西哥人群中抗溶组织内阿米巴抗体的流行情况进行了研究。抗原是通过用Triton X - 100从溶组织内阿米巴HM1 - IMSS无菌培养物中提取获得的膜蛋白混合物。通过比较阿米巴肝脓肿患者和健康志愿者的血清样本对该方法进行了标准化。在国家血清流行病学调查提供的60538份样本中，发现抗体阳性率为4.49%（95%置信限为4.32 - 4.65%）。该国中部地区出现的抗体效价更高。女性与男性的比例为1.25:1。生活在大都市地区的人群可能比生活在农村的人群感染年龄更小。与在相同样本框架下使用间接血凝试验（IHA）的一项研究相比，ELISA检测得到的血清阳性率存在重要差异。
