Isaac D D, Andrew D J
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205-2196, USA.
Genes Dev. 1996 Jan 1;10(1):103-17. doi: 10.1101/gad.10.1.103.
The trachealess (trh) gene of Drosophila is required for embryonic tube formation. In trh mutants, tube-forming cells of the salivary gland, trachea, and filzkörper fail to invaginate to form tubes and remain on the embryo surface. We identified a P-element insertion that disrupts trh function and used the insert to clone and characterize trh. trh is expressed in the salivary duct, trachea, and filzköper primordia, and expression persists in these cells throughout embryogenesis. trh expression in the salivary duct is controlled by the homeotic gene, Sex combs reduced (Scr), and by another salivary gland gene, fork head (fkh). trh is homologous to two transcription factors: the human hypoxia-inducible factor-1 alpha and the Drosophila Single-minded protein.
果蝇的无气管（trh）基因是胚胎管形成所必需的。在trh突变体中，唾液腺、气管和丝状器的管形成细胞无法内陷形成管子，而是留在胚胎表面。我们鉴定出一个破坏trh功能的P因子插入，并利用该插入片段克隆和表征trh。trh在唾液导管、气管和丝状器原基中表达，并且在整个胚胎发育过程中这些细胞中持续表达。唾液导管中trh的表达受同源异型基因“减少性梳”（Scr）以及另一个唾液腺基因“叉头”（fkh）的控制。trh与两种转录因子同源：人类缺氧诱导因子-1α和果蝇的单 minded蛋白。
