Ravn P, Linnet J, Klokker M, Pedersen B K
Department of Infectious Diseases, Rigshospitalet, Copenhagen, Denmark.
Immunopharmacology. 1995 Sep;30(3):247-53. doi: 10.1016/0162-3109(95)00029-s.
The effect of isoniazid on proliferative response, natural killer (NK) cell activity and lymphocyte subset distribution of blood mononuclear cells (BMNC) was investigated. To evaluate the effect of treatment with isoniazid in pharmacologic concentrations, twenty healthy HIV-seronegative volunteers were randomized into two groups: one group received isoniazid tablets plus pyridoxin tablets once a day for 30 days, the other group received pyridoxin only. Blood samples were collected on day 0 and day 30. Inhibition of the PHA-induced proliferative response was demonstrated in lymphocyte cultures from isoniazid-treated volunteers (p < 0.001). However, no effect was seen on the IL-2- or antigen (PPD)-induced proliferative response or the NK cell activity of isolated BMNC. Inhibition of the PHA-induced proliferative response could not be related to changes in the distribution of CD3+, CD4+, CD8+, CD14, or CD19+ lymphocyte subsets. The effects, in vitro, were investigated by addition of isoniazid to cultures of BMNC isolated from either HIV-seroposive or HIV-seronegative donors who did not receive any treatment. We found that isoniazid did not influence the mitogen- or antigen-stimulated proliferative response or the NK cell activity.
研究了异烟肼对血液单核细胞（BMNC）增殖反应、自然杀伤（NK）细胞活性及淋巴细胞亚群分布的影响。为评估药理浓度异烟肼治疗的效果，将20名健康的HIV血清阴性志愿者随机分为两组：一组每天服用一次异烟肼片加维生素B6片，共30天，另一组仅服用维生素B6。在第0天和第30天采集血样。在接受异烟肼治疗的志愿者的淋巴细胞培养物中，PHA诱导的增殖反应受到抑制（p<0.001）。然而，对IL-2或抗原（PPD）诱导的增殖反应或分离的BMNC的NK细胞活性未见影响。PHA诱导的增殖反应受到抑制与CD3+、CD4+、CD8+、CD14或CD19+淋巴细胞亚群分布的变化无关。通过向未接受任何治疗的HIV血清阳性或HIV血清阴性供体分离的BMNC培养物中添加异烟肼来研究体外效应。我们发现异烟肼不影响有丝分裂原或抗原刺激的增殖反应或NK细胞活性。
