Nielsen H B, Secher N H, Kappel M, Pedersen B K
The Copenhagen Muscle Research Center, Departments of Infectious Diseases and Anesthesia, University of Copenhagen, DK-2200 Copenhagen, Denmark.
Am J Physiol. 1998 Oct;275(4):R1227-31. doi: 10.1152/ajpregu.1998.275.4.R1227.
This study evaluated whether N-acetylcysteine (NAC) attenuates the reduced lymphocyte proliferation and natural killer (NK) cell activity responses to exercise in humans. Fourteen oarsmen were double-blind randomized to either NAC (6 g daily for 3 days) or placebo groups. During 6-min "all-out" ergometer rowing, the concentration of lymphocytes in the peripheral blood increased, with no significant difference between NAC and placebo as reflected in lymphocyte subsets: CD4(+), CD8(+), CD16(+), and CD19(+) cells. The phytohemagglutinin-stimulated lymphocyte proliferation decreased from 9,112 +/- 2,865 to 5,851 +/- 1,588 cpm (P < 0.05), but it was not affected by NAC. During exercise, the NK cell activity was elevated from 17 +/- 3 to 38 +/- 4% and it decreased to 7 +/- 1% below the resting value 2 h into recovery. Yet, when evaluated as lytic units per CD16(+) cell, the NK cell activity decreased during and after exercise without a significant effect of NAC. We conclude that NAC does not attenuate the reduction in lymphocyte proliferation and NK cell activity associated with intense exercise.
本研究评估了N-乙酰半胱氨酸（NAC）是否能减轻人体运动后淋巴细胞增殖减少和自然杀伤（NK）细胞活性降低的反应。14名赛艇运动员被双盲随机分为NAC组（每天6克，共3天）或安慰剂组。在6分钟的“全力”测力计划船过程中，外周血淋巴细胞浓度升高，NAC组和安慰剂组在淋巴细胞亚群（CD4(+)、CD8(+)、CD16(+)和CD19(+)细胞）方面无显著差异。植物血凝素刺激的淋巴细胞增殖从9,112±2,865 cpm降至5,851±1,588 cpm（P<0.05），但不受NAC影响。运动期间，NK细胞活性从17±3升高至38±4%，恢复2小时后降至低于静息值7±1%。然而，以每CD16(+)细胞的溶解单位来评估时，运动期间和运动后NK细胞活性降低，NAC无显著影响。我们得出结论，NAC不能减轻与剧烈运动相关的淋巴细胞增殖减少和NK细胞活性降低。
