Nakamura M, Ito Y, Ogawa K, Michisuji Y, Sato S, Takada M, Hayashi M, Yaginuma S, Yamamoto S
Institute for Life Science Research, Asahi Chemical Industry Co., Ltd., Shizuoka, Japan.
J Antibiot (Tokyo). 1995 Dec;48(12):1389-95. doi: 10.7164/antibiotics.48.1389.
Stachybocins A, B and C, novel endothelin (ET) receptor antagonists, were isolated from the culture filtrate of Stachybotrys sp. M6222. They were extracted with ethyl acetate and then purified by alumina and silica gel column chromatographies. The molecular formulae of stachybocins were determined to be C52H70N2O10 (stachybocin A) and C52H70N2O11 (stachybocins B and C). It was supposed that they consisted of spirobenzofuran and terpene units from NMR spectra. They showed the inhibitory activity of 125I-ET-1 binding to rate ETA, human ETA and human ETB receptors.
从链格孢属菌株M6222的培养滤液中分离出新型内皮素（ET）受体拮抗剂水苏毒素A、B和C。它们用乙酸乙酯萃取，然后通过氧化铝和硅胶柱色谱法进行纯化。水苏毒素的分子式确定为C52H70N2O10（水苏毒素A）和C52H70N2O11（水苏毒素B和C）。根据核磁共振光谱推测，它们由螺苯并呋喃和萜烯单元组成。它们显示出对125I-ET-1与大鼠ETA、人ETA和人ETB受体结合的抑制活性。
