Matar K M, Nicholls P J, al-Hassan M I, Tekle A
Department of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
J Clin Pharm Ther. 1995 Aug;20(4):229-34. doi: 10.1111/j.1365-2710.1995.tb00654.x.
A rapid method for the simultaneous determination of oxcarbazepine (OXC) and its active metabolite (10-hydroxycarbazepine) in human and rat plasma by reversed phase high-performance liquid chromatography is described. The method involves a simple one-step extraction of the drugs from plasma with dichloromethane. The extract was evaporated and the residue was reconstituted with mobile phase and injected onto a Novapak C18 column. The eluting solvent was 20% acetonitrile in water at a flow rate of 1.5 ml/min and the detector was monitored at 215 nm. The detection limit of OXC and 10-hydroxycarbazepine was 50 and 20 ng/ml, respectively. The within-day and between-day coefficients of variation for OXC and its active metabolite were 2.57-6.95% and 4.21-8.3%, respectively. The relative and absolute recoveries varied between 71.4% and 104.0%. The applicability of the analytical procedure to pharmacokinetic studies was illustrated.
本文描述了一种通过反相高效液相色谱法同时测定人和大鼠血浆中奥卡西平（OXC）及其活性代谢物（10-羟基奥卡西平）的快速方法。该方法包括用二氯甲烷从血浆中简单一步提取药物。提取物蒸发后，残渣用流动相复溶并注入到NovaPak C18柱上。洗脱溶剂为20%乙腈水溶液，流速为1.5 ml/min，在215 nm处进行检测。奥卡西平和10-羟基奥卡西平的检测限分别为50 ng/ml和20 ng/ml。奥卡西平及其活性代谢物的日内和日间变异系数分别为2.57 - 6.95%和4.21 - 8.3%。相对回收率和绝对回收率在71.4%至104.0%之间。文中说明了该分析方法在药代动力学研究中的适用性。
