Pienimäki P, Fuchs S, Isojärvi J, Vähäkangas K
Department of Pharmacology and Toxicology, University of Oulu, Finland.
J Chromatogr B Biomed Appl. 1995 Nov 3;673(1):97-105. doi: 10.1016/0378-4347(95)00246-f.
An HPLC assay for carbamazepine or oxcarbazepine (OXC) and six of their metabolites in one run was applied to 35 clinical samples from patients receiving monotherapy. This rapid and economical method, utilizing a simple one-step extraction with methyl tert.-butyl ether before the run, showed recoveries of 77-108%, except for 43% for 10,11-trans-dihydroxy-10,11-dihydrocarbamazepine, from 500-microliters samples, with detection limits of 8-12 ng/ml and limits of quantification of 14-55 ng/ml depending on the compound. Indication of a new OXC metabolite, 3-hydroxycarbamazepine, was found in 2/12 patients.
一种可在一次运行中同时检测卡马西平或奥卡西平（OXC）及其六种代谢物的高效液相色谱法应用于35份接受单一疗法患者的临床样本。这种快速且经济的方法，在运行前使用甲基叔丁基醚进行简单的一步萃取，从500微升样本中获得的回收率为77%-108%，10,11-反式二羟基-10,11-二氢卡马西平除外，其回收率为43%，根据化合物不同，检测限为8-12纳克/毫升，定量限为14-55纳克/毫升。在12名患者中的2名患者体内发现了一种新的奥卡西平代谢物3-羟基卡马西平。
