Imai A, Horibe S, Fuseya S, Iida K, Takagi H, Tamaya T
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
J Med. 1995;26(3-4):163-74.
The herbal medicine shakuyaku-kanzo-to has been used for the treatment of dysmenorrhea. We attempted to establish the effects of shakuyaku-kanzo-to on prostaglandin (PG) levels and the turnover of arachidonic acid in endometrial cells. Human endometrial cells isolated from proliferative-phase endometria were examined. Arachidonic acid reacylation activity was determined by [3H]arachidonic acid incorporation into endometrial cell phospholipid. Its deacylation rate was determined by both 3H-liberation from [3H]-arachidonic acid-labelled phospholipids and production of lyso-phospholipid. [3H]-PG levels were also analyzed. Shakuyaku-kanzo-to enhanced the rate and maximal level of arachidonic acid incorporation by 1.5-fold into phosphatidyl-ethanolamine and phosphatidylcholine. Degradation of the phospholipids to liberate arachidonic acid and lyso-derivatives was not affected by the shakuyaku-kanzo-to. Shakuyaku-kanzo-to decreased PG levels by approximately 50% of control. Because shakuyaku-kanzo-to stimulates arachidonic acid esterification to phospholipids, it seems likely that shakuyaku-kanzo-to depletes cellular free arachidonic acid with a consequent suppression of prostaglandin synthesis. Shakuyaku-kanzo-to may exert its action against dysmenorrhea through preventing prostaglandin production.
中药芍药甘草汤已被用于治疗痛经。我们试图确定芍药甘草汤对子宫内膜细胞中前列腺素（PG）水平和花生四烯酸代谢的影响。对从增殖期子宫内膜分离出的人子宫内膜细胞进行了检测。通过将[3H]花生四烯酸掺入子宫内膜细胞磷脂中来测定花生四烯酸再酰化活性。其脱酰化率通过[3H] - 花生四烯酸标记的磷脂中3H的释放以及溶血磷脂的产生来确定。还分析了[3H] - PG水平。芍药甘草汤使花生四烯酸掺入磷脂酰乙醇胺和磷脂酰胆碱的速率和最大水平提高了1.5倍。芍药甘草汤对磷脂降解以释放花生四烯酸和溶血衍生物没有影响。芍药甘草汤使PG水平降低至对照的约50％。由于芍药甘草汤刺激花生四烯酸酯化到磷脂中似乎很可能芍药甘草汤消耗细胞内游离花生四烯酸，从而抑制前列腺素合成。芍药甘草汤可能通过阻止前列腺素产生来发挥其对痛经的作用。
