Löfgren S, Söderberg P G
Research Department, St. Erik Eye Hospital, Stockholm, Sweden.
J Photochem Photobiol B. 1995 Oct;30(2-3):145-51. doi: 10.1016/1011-1344(95)07183-3.
UV radiation and short wavelength visible light are known to damage various tissues in the eye. This paper investigates the effect on rat lens glycolysis after in vivo exposure with 90 kJ m-2 narrow band UV radiation (UVB, 300 nm) and 90 kJ m-2 blue light (435 nm) radiation. After exposure, all lenses were incubated in Medium 199. Samples of culture medium were withdrawn after 2, 4, 6 h and 5, 10, 20 h in two UVB studies and after 5, 10 and 20 h in a blue light study. Lactate is the major end product of lens glycolysis. Lactate was determined with a modified enzymatic-photometric method. Intralenticular lactate was determined in one UVB experiment. In the UVB experiments we found a lower lactate production in the exposed lenses 2-6 h after exposure. There was an accumulation of lactate inside UVB-exposed lenses after 6 h incubation compared with their contralateral lenses. No significant effect on lactate production was observed in the blue light experiment. CONCLUSIONS. UVB induced a reversible inhibition of glycolysis. UVB also induced an accumulation of lactate inside the lens. Blue light tended to increase glycolysis.
已知紫外线辐射和短波长可见光会损害眼睛中的各种组织。本文研究了用90 kJ m-2窄带紫外线辐射（UVB，300 nm）和90 kJ m-2蓝光（435 nm）进行体内照射后对大鼠晶状体糖酵解的影响。照射后，所有晶状体均在199培养基中孵育。在两项UVB研究中，分别在2、4、6小时以及5、10、20小时后取出培养基样本；在蓝光研究中，则在5、10和20小时后取出样本。乳酸是晶状体糖酵解的主要终产物。采用改良的酶促光度法测定乳酸含量。在一项UVB实验中测定了晶状体内部的乳酸含量。在UVB实验中，我们发现照射后2至6小时，受照射的晶状体中乳酸生成量较低。与对侧晶状体相比，孵育6小时后，UVB照射的晶状体内部出现了乳酸积累。在蓝光实验中未观察到对乳酸生成有显著影响。结论：UVB诱导了糖酵解的可逆抑制。UVB还诱导了晶状体内部乳酸的积累。蓝光倾向于增加糖酵解。
