Spillert C R, Lee C S, Federici E E, Lazaro E J
Department of Surgery, UMDNJ-New Jersey Medical School, Newark 07103, USA.
J Natl Med Assoc. 1995 Dec;87(12):869-71.
There are two important reasons why most platelet function studies can be inaccurate. First, platelet function deteriorates when blood is taken out of the vascular tree. Second, tests performed on platelets removed from the blood do not incorporate the role of other cellular and chemical components that may alter platelet activity. This article demonstrates that a coagulation test developed in our laboratory can accurately assess the role of platelet age on the speed of the coagulation of blood. Samples (5.0 mL) of citrated venous blood from 15 volunteers were divided into two groups. One group (n = 6), comprised of subgroups A, B, C, and D of 950 microL aliquots each, was tested within 3 hours. The second group (n = 9), comprised of subgroups E, F, G, and H of 950 microL aliquots each, was tested at 24 hours. The aliquots were added to 50 microL saline without collagen (subgroups A and E), 50 microL saline with 10 micrograms collagen (subgroups B and F), 50 microL saline with 50 micrograms collagen (subgroups C and G), and 50 microL saline with 100 micrograms collagen (subgroups D and H). All collagen-incubated fresh blood samples were significantly more hypercoagulable (shorter recalcification times) compared with the control (no collagen) blood. In the 24-hour-old blood, changes were significant only in the sample with 50 micrograms/mL collagen. We conclude that these data authenticate the role of platelet age in the assessment of the coagulation process.
大多数血小板功能研究可能不准确，原因主要有两个。其一，血液从血管系统中抽出后，血小板功能会恶化。其二，对从血液中分离出的血小板进行的检测没有纳入其他可能改变血小板活性的细胞和化学成分的作用。本文表明，我们实验室开发的一种凝血测试能够准确评估血小板年龄对血液凝固速度的作用。从15名志愿者采集的5.0 mL枸橼酸抗凝静脉血样本被分为两组。第一组（n = 6），由分别含950微升等分试样的A、B、C和D亚组组成，在3小时内进行检测。第二组（n = 9），由分别含950微升等分试样的E、F、G和H亚组组成，在24小时时进行检测。将等分试样分别加入50微升不含胶原蛋白的生理盐水（A和E亚组）、50微升含10微克胶原蛋白的生理盐水（B和F亚组）、50微升含50微克胶原蛋白的生理盐水（C和G亚组）以及50微升含100微克胶原蛋白的生理盐水（D和H亚组）中。与对照（不含胶原蛋白）血液相比，所有含胶原蛋白孵育的新鲜血液样本的血液凝固性均显著更高（复钙时间更短）。在24小时的血液中，仅含50微克/毫升胶原蛋白的样本有显著变化。我们得出结论，这些数据证实了血小板年龄在凝血过程评估中的作用。
