一种参与病毒诱导的IFN-A基因转录的新型PRD I和TG结合活性。

Génin P, Bragança J, Darracq N, Doly J, Civas A

Laboratoire de Régulation de l'Expression des Gènes Eucaryotes, CNRS, UPR 37, UFR Biomédicale des Saints-Pères, Université René Descartes, Paris, France.

Nucleic Acids Res. 1995 Dec 25;23(24):5055-63. doi: 10.1093/nar/23.24.5055.

Comparative analysis of the inducible elements of the mouse interferon A4 and A11 gene promoters (IE-A4 and IE-A11) by transient transfection experiments, DNase 1 footprinting and electrophoretic mobility shift assays resulted in identification of a virus-induced binding activity suggested to be involved in NDV-induced activation of transcription of these genes. The virus-induced factor, termed VIF, is activated early by contact of virions with cells. It specifically recognizes the PRD I-like domain shared by both inducible elements, as well as the TG-like domain of IE-A4. This factor, distinct from the IRF-1, IRF-2 and the alpha F1 binding proteins and presenting a different affinity pattern from that of the TG protein, is proposed as a candidate for IFN-type I gene regulation.

通过瞬时转染实验、DNA酶I足迹法和电泳迁移率变动分析对小鼠干扰素A4和A11基因启动子（IE-A4和IE-A11）的诱导元件进行比较分析，结果鉴定出一种病毒诱导的结合活性，提示其参与新城疫病毒诱导的这些基因转录激活。这种病毒诱导因子称为VIF，通过病毒粒子与细胞接触早期被激活。它特异性识别两个诱导元件共有的PRD I样结构域以及IE-A4的TG样结构域。该因子不同于IRF-1、IRF-2和αF1结合蛋白，且呈现出与TG蛋白不同的亲和模式，被认为是I型干扰素基因调控的候选因子。