Janes S L, Kyle P M, Redman C, Goodall A H
Academic Department of Haematology, Royal Free Hospital School of Medicine, London, UK.
Thromb Haemost. 1995 Oct;74(4):1059-63.
Pre-eclampsia is a common complication of pregnancy, in which platelets may have an early pathogenetic role. In this prospective study a whole blood flow cytometric method has been used to detect circulating activated platelets in pregnant women prior to the development of pre-eclampsia. Activated platelets were identified by bound fibrinogen or by CD63 antigen expression. Of 121 healthy primiparous women studied at 28 weeks of pregnancy, 18 (15%) developed clinical pre-eclampsia six to thirteen weeks later. The platelets of these women showed increased fibrinogen binding ex vivo (5.1% platelets positive, compared with 3.4% in those who completed a normal pregnancy, p < 0.02), and increased CD63 antigen expression (0.73% positive compared to 0.45%, p = 0.01). In contrast, no differences between the women with different outcomes were detected at 28 weeks in platelet counts, or plasma beta-thromboglobulin levels. These findings confirm that whole blood flow cytometry is a sensitive technique for investigating platelet activation in a clinical setting and support the hypothesis that platelets have a critical role in the pathogenesis of pre-eclampsia.
子痫前期是妊娠期常见的并发症，血小板可能在其早期发病机制中起作用。在这项前瞻性研究中，采用全血流式细胞术检测子痫前期发生前孕妇循环中的活化血小板。通过结合的纤维蛋白原或CD63抗原表达来识别活化血小板。在121名妊娠28周时接受研究的健康初产妇中，18名（15%）在6至13周后发生了临床子痫前期。这些女性的血小板在体外显示出纤维蛋白原结合增加（5.1%的血小板呈阳性，而正常妊娠者为3.4%，p<0.02），且CD63抗原表达增加（0.73%呈阳性，而正常妊娠者为0.45%，p = 0.01）。相比之下，不同结局的女性在妊娠28周时血小板计数或血浆β-血小板球蛋白水平未检测到差异。这些发现证实全血流式细胞术是临床研究血小板活化的敏感技术，并支持血小板在子痫前期发病机制中起关键作用这一假说。
