Hildén J O, Gottvall T, Lindblom B
Department of Transfusion Medicine & Clinical Immunology, University Hospital, Linköping, Sweden.
Tissue Antigens. 1995 Oct;46(4):313-5. doi: 10.1111/j.1399-0039.1995.tb02498.x.
Blood samples from 24 Rh(D) immunized women were analyzed for antibody titers and quantification of anti-D. The HLA-DR and -DQ polymorphisms were identified as RFLP. In 11 women with titers 16-256 the HLA-DQB1 allele *0201 was found in 18%, i e as in a reference population. In 13 women with titers > or = 512 the HLA-DQB1 allele *0201 was found in 85% indicating a correlation between severe Rh(D) immunization with high titers/quantification values and the DQB1 allele *0201. In this group the fetus was severely affected by the immunization and treatment during pregnancy was frequently needed. HLA phenotyping of women known to have anti-D antibodies early in pregnancy seems to be an effective way to assess the probability of severe hemolytic disease of the newborn.
对24名Rh(D)免疫的女性的血样进行了抗体滴度分析和抗-D定量分析。HLA-DR和-DQ多态性通过限制性片段长度多态性（RFLP）鉴定。在11名滴度为16 - 256的女性中，HLA-DQB1等位基因0201的出现率为18%，即与参考人群中的出现率相同。在13名滴度≥512的女性中，HLA-DQB1等位基因0201的出现率为85%，这表明高滴度/定量值的严重Rh(D)免疫与DQB1等位基因*0201之间存在相关性。在这组人群中，胎儿受到免疫的严重影响，孕期经常需要进行治疗。对已知在妊娠早期具有抗-D抗体的女性进行HLA表型分析似乎是评估新生儿严重溶血病可能性的一种有效方法。
