Kajon A E, Wadell G
Department of Virology University of Umeå, Sweden.
Virology. 1996 Jan 15;215(2):190-6. doi: 10.1006/viro.1996.0022.
Adenovirus type 7h is currently the predominant virulent genome type of serotype 7 isolated in Argentina, Chile, and Uruguay in association with severe infantile pneumonia. In order to characterize possible molecular determinants of pathogenicity, the nucleotide sequence of a 5904-bp fragment (76 to 93 mu) containing the entire E3 region and the fiber gene of Ad7h was established. The organization of the ORFs within the E3 region was similar to that reported for the prototype strains of Ad7 and Ad3. A comparison of the nucleotide and amino acid sequences of all ORFs revealed a higher homology between Ad7h and Ad7p than between Ad7h and Ad3 for 12.0K and 16.1K, whereas the 15.3K ORF and the adjacent fiber gene were strikingly more homologous to those of Ad3 (99.5 vs 81.1% and 98.2 vs 66.6%, respectively). The equivalent to ORF 7.7K in Ad7p was missing in Ad7h due to a deletion and a mutation affecting the start codon (ATG-->ATT). Although the hemagglutinin of the Ad7h fiber could not be characterized due to its lack of activity on monkey erythrocytes, our results indicate that Ad7h is an intermediate strain 7-3.
7h型腺病毒是目前在阿根廷、智利和乌拉圭分离出的与严重婴儿肺炎相关的7型血清型的主要致病基因组类型。为了确定可能的致病性分子决定因素，我们确定了包含Ad7h整个E3区域和纤维基因的5904bp片段（76至93 mu）的核苷酸序列。E3区域内开放阅读框（ORF）的组织与Ad7和Ad3原型菌株报道的相似。所有ORF的核苷酸和氨基酸序列比较显示，在12.0K和16.1K方面，Ad7h与Ad7p之间的同源性高于Ad7h与Ad3之间的同源性，而15.3K ORF和相邻的纤维基因与Ad3的同源性明显更高（分别为99.5%对81.1%和98.2%对66.6%）。由于缺失和影响起始密码子（ATG→ATT）的突变，Ad7h中缺少Ad7p中相当于7.7K ORF的序列。尽管由于Ad7h纤维的血凝素对猴红细胞缺乏活性而无法对其进行表征，但我们的结果表明Ad7h是一种7-3中间菌株。
