Reig S, Buée-Scherrer V, Mourton-Gilles C, Defossez A, Delacourte A, Beauvillain J C, Mazzuca M
INSERM Unité 422, Lille, France.
Acta Neuropathol. 1995;90(5):441-7. doi: 10.1007/BF00294803.
Senile plaque and paired helical filament (PHF) formation are characteristic of Alzheimer's disease, but the mechanisms leading to these lesions still remain unclear. To understand them better, we have performed different immunolabellings of amyloid protein and PHF. We describe a very specific immunodetection of PHF with AD2, a monoclonal antibody directed against a hyperphosphorylated epitope of PHF-tau, and use double immunolabelling to show that PHF and plaque amyloid are discretely labelled by different antibodies. We also discuss different mechanisms of PHF maturation.
老年斑和双螺旋丝（PHF）的形成是阿尔茨海默病的特征，但导致这些病变的机制仍不清楚。为了更好地理解它们，我们对淀粉样蛋白和PHF进行了不同的免疫标记。我们描述了用AD2对PHF进行非常特异性的免疫检测，AD2是一种针对PHF-tau高磷酸化表位的单克隆抗体，并使用双重免疫标记来显示PHF和斑块淀粉样蛋白被不同抗体分别标记。我们还讨论了PHF成熟的不同机制。
