Drubin D G, Caput D, Kirschner M W
J Cell Biol. 1984 Mar;98(3):1090-7. doi: 10.1083/jcb.98.3.1090.
Tau protein is a collection of closely related polypeptides that associate with microtubules in vivo and stimulate their assembly in vitro. Using an affinity-purified antiserum against bovine brain tau protein, we found that the number and amount of tau polypeptides changes dramatically during mouse brain development. The different forms appear to result from changes in tau mRNA since in vitro translation products reflect the qualitative and quantitative changes found in vivo. To study the mRNA and genomic complexity of tau protein, we used tau mRNA, purified from polysomes with tau antiserum, to isolate embryonic mouse tau complementary DNA clones. With these probes we have determined that embryonic tau protein is translated from a 6-kb mRNA that persists throughout brain development.
tau蛋白是一组密切相关的多肽，在体内与微管结合，并在体外刺激其组装。使用针对牛脑tau蛋白的亲和纯化抗血清，我们发现tau多肽的数量和含量在小鼠脑发育过程中发生了显著变化。不同的形式似乎是由于tau mRNA的变化，因为体外翻译产物反映了体内发现的定性和定量变化。为了研究tau蛋白的mRNA和基因组复杂性，我们使用用tau抗血清从多核糖体中纯化的tau mRNA来分离胚胎小鼠tau互补DNA克隆。利用这些探针，我们确定胚胎tau蛋白是由一种6kb的mRNA翻译而来的，这种mRNA在整个脑发育过程中持续存在。
