在被有效感染的猴细胞中猿猴病毒40的RNA：去核细胞中RNA的合成与降解动力学
RNAs of simian virus 40 in productively infected monkey cells: kinetics of formation and decay in enucleate cells.
作者信息
Aloni Y, Shani M, Reuveni Y
出版信息
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2587-91. doi: 10.1073/pnas.72.7.2587.
Abstract
We demonstrate here the usefulness of cytochalasin B enucleate cells for the study of the metabolism of cytoplasmic mRNA and for determining its half-life in animal cells. Simian virus 40 infected monkey cells in which the RNA had been labeled with [3H]uridine were enucleated, and the decay of the two prominent RNAs of simian virus 40, the 19S and 16S species, was measured by analysis on sucrose gradients. The results of these experiments, together with kinetic analysis of nuclear and cytoplasmic viral RNA, indicate a precursor-product relationship between the 19S and 16S cytoplasmic viral RNA species, which decay by first-order kinetics with a mean half-life of about 3 hr and 6 hr, respectively.
摘要
我们在此证明，细胞松弛素B去核细胞对于研究细胞质mRNA的代谢及其在动物细胞中的半衰期是有用的。用[3H]尿苷标记RNA的猴空泡病毒40感染的猴细胞被去核，通过蔗糖梯度分析来测量猴空泡病毒40的两种主要RNA即19S和16S种类的衰变。这些实验的结果，连同对核和细胞质病毒RNA的动力学分析，表明19S和16S细胞质病毒RNA种类之间存在前体-产物关系，它们分别以一级动力学衰变，平均半衰期约为3小时和6小时。
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RNAs of simian virus 40 in productively infected monkey cells: kinetics of formation and decay in enucleate cells.在被有效感染的猴细胞中猿猴病毒40的RNA：去核细胞中RNA的合成与降解动力学
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