Pascual N, Bellés X, Delbecque J P, Hua Y J, Koolman J
Department of Agrobiology, Centro de Investigación y Desarrollo (C.S.I.C.), Barcelona, Spain.
Z Naturforsch C J Biosci. 1995 Nov-Dec;50(11-12):862-7. doi: 10.1515/znc-1995-11-1219.
The performance of enzyme immunoassay (EIA) and radioimmunoassay (RIA) in the quantitative analysis of ecdysteroids was compared. The EIA was found to be at least equivalent to the RIA with respect to analytical range and sensitivity and to be more comfortable with respect to safety and time saving. When biological samples were analyzed by both assays a good correlation (r = 0.83) was found. Since the EIA has certain advantages over the RIA, we now recommend the use of the former assay for the quantification of ecdysteroids.
比较了酶免疫测定法（EIA）和放射免疫测定法（RIA）在蜕皮类固醇定量分析中的性能。发现EIA在分析范围和灵敏度方面至少与RIA相当，并且在安全性和节省时间方面更具优势。当用这两种测定法分析生物样品时，发现具有良好的相关性（r = 0.83）。由于EIA相对于RIA具有某些优势，我们现在推荐使用前一种测定法进行蜕皮类固醇的定量分析。
