Kaplan L A, Chen I W, Gau N, Fearn J, Maxon H, Volle C, Stein E A
Clin Biochem. 1981 Aug;14(4):182-6. doi: 10.1016/s0009-9120(81)91212-1.
We have compared three analytical systems for the measurement of serum thyroxine: enzyme-linked immunoassay (EIA), fluorescent immunoassay (FIA) and a radioimmunoassay (RIA). These were evaluated with respect to their precision, accuracy, analytical sensitivity and sample throughput. The RIA is more sensitive than the EIA (10 microgram/L vs. 35 Ug/L. Both systems have excellent precision (X = 86 ug/L, C.V.RIA = C.V.EIA = 4.6%). Both the EIA and RIA demonstrate good accuracy with recovery of between 97-98% of added thyroxine. The FIA has an apparent sensitivity between that of the RIA and EIA (25 microgram/L), but a precision consistently lower than the other two systems (C.V. = 7.4%, X = 86 microgram/L). Patients' results by RIA compared well with those from EIA )r = 0.91, P less than 0.05), but a poor correlation was found with FIA (r = 0.865, P greater than 0.05). Although not fully automated, the EIA performed on the Abbott ABA-100 analyzer has a sample throughput equal to the automated RIA system (Micromedic, Concept 4).
酶联免疫吸附测定（EIA）、荧光免疫测定（FIA）和放射免疫测定（RIA）。对这些系统的精密度、准确性、分析灵敏度和样本通量进行了评估。RIA比EIA更灵敏（分别为10微克/升和35微克/升）。两种系统都具有出色的精密度（X = 86微克/升，RIA的变异系数 = EIA的变异系数 = 4.6%）。EIA和RIA的准确性都很好，添加甲状腺素的回收率在97%至98%之间。FIA的表观灵敏度介于RIA和EIA之间（25微克/升），但其精密度始终低于其他两个系统（变异系数 = 7.4%，X = 86微克/升）。RIA检测患者的结果与EIA的结果相关性良好（r = 0.91，P < 0.05），但与FIA的相关性较差（r = 0.865，P > 0.05）。虽然不是全自动的，但在雅培ABA - 100分析仪上进行的EIA的样本通量与自动化RIA系统（Micromedic，Concept 4）相当。
