Rastelli L, Richman R, Kuroda M I
Howard Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine, Houston, TX 77030, USA.
Mech Dev. 1995 Oct;53(2):223-33. doi: 10.1016/0925-4773(95)00438-7.
We have analyzed the expression pattern and localization of MLE, MLS-1, MSL-2, and histone H4Ac16 during embryogenesis to determine when msl-dependent dosage compensation begins. Maternal MSL-1 and MLE are present in both sexes at fertilization. MSL-2 lacks a maternal component, and male-specific zygotic expression is detectable at the end of blastoderm. During germ band extension, MSL-1, MSL-2, MLE, and histone H4Ac16 display coincident sub-nuclear localization in male embryos. In embryos lacking one of the MSL proteins, the sub-nuclear localization of the other MSLs and of histone H4Ac16 is not detected. We conclude that the MSL proteins associate with the X chromosome and are interdependent since early embryogenesis.
我们分析了MLE、MLS-1、MSL-2和组蛋白H4Ac16在胚胎发生过程中的表达模式和定位，以确定msl依赖的剂量补偿何时开始。受精时，母源MSL-1和MLE在两性中均存在。MSL-2缺乏母源成分，在胚盘末期可检测到雄性特异性合子表达。在胚带延伸期间，MSL-1、MSL-2、MLE和组蛋白H4Ac16在雄性胚胎中显示出一致的亚核定位。在缺乏一种MSL蛋白的胚胎中，未检测到其他MSL和组蛋白H4Ac16的亚核定位。我们得出结论，自胚胎早期发生起，MSL蛋白就与X染色体相关联且相互依赖。
