Panasenko O M, Arnhold J, Vladimirov Iu A, Sergienko V I
Institute of Medical Physics and Biophysics, Leipzig University, Germany.
Biokhimiia. 1995 Sep;60(9):1419-28.
The chemiluminescence in the presence of luminol has been used to measure the amount of hypochlorite and its reduction during the interaction with oxidized and non-oxidized liposomes from egg yolk phosphatidylcholine as well as with organic peroxides (tert-butylhydroperoxide, cumene hydroperoxide, di-tert-butylperoxide, tert-butylperbenzoate, di-benzoylperoxide), and epoxides (cis- and trans-2,3-epoxy-butane, cholesterol-5 alpha,6 alpha-epoxide, and cis-9,10-epoxystearic acid). Since hypochlorite did not react with the saturated phospholipid, dimyristoylphosphatidylcholine (DMPC), and the reduction of double bonds in egg yolk phosphatidylcholine liposomes occurred at the same rate in both oxidized and non-oxidized liposomes, it may be suggested that hypochlorite interacted precisely with LPO products. None of the epoxides tested in this study, similar to di-tert-butylperoxide, tert-butylperbenzoate, di-benzoylperoxide, incorporated into liposomes reacted with hypochlorite. In contrast, tert-butylhydroperoxide and cumene hydroperoxide effectively reacted with it. The data obtained suggest that epoxides, dialkyl-, diacyl-, and alkyl-acyl-peroxides are not involved in hypochlorite-induced LPO. At the same time, organic hydroperoxides commonly present in certain amounts in the biomembrane lipid phase in vivo may play a role lf an intermediate; its interaction with HOCl/OCl- gives rise to free radical formation followed by accumulation of LPO products.
在鲁米诺存在的情况下，化学发光已被用于测量次氯酸盐的含量及其在与来自蛋黄磷脂酰胆碱的氧化和未氧化脂质体以及有机过氧化物（叔丁基过氧化氢、异丙苯过氧化氢、二叔丁基过氧化物、叔丁基过苯甲酸酯、二苯甲酰过氧化物）和环氧化物（顺式和反式-2,3-环氧丁烷、胆固醇-5α,6α-环氧化物和顺式-9,10-环氧硬脂酸）相互作用过程中的还原情况。由于次氯酸盐不与饱和磷脂二肉豆蔻酰磷脂酰胆碱（DMPC）反应，并且蛋黄磷脂酰胆碱脂质体中双键的还原在氧化和未氧化脂质体中的速率相同，因此可以认为次氯酸盐恰好与脂质过氧化产物相互作用。本研究中测试的环氧化物，类似于二叔丁基过氧化物、叔丁基过苯甲酸酯、二苯甲酰过氧化物，掺入脂质体后均不与次氯酸盐反应。相反，叔丁基过氧化氢和异丙苯过氧化氢能与之有效反应。所获得的数据表明，环氧化物、二烷基过氧化物、二酰基过氧化物和烷基酰基过氧化物不参与次氯酸盐诱导的脂质过氧化。同时，体内生物膜脂质相中通常存在一定量的有机氢过氧化物可能作为中间体发挥作用；它与HOCl/OCl-的相互作用会产生自由基形成，随后脂质过氧化产物积累。
