Khlebodarova T M, Malchenko S N, Matveeva N M, Pack S D, Sokolova O V, Alabiev B Y, Belousov E S, Peremislov V V, Nayakshin A M, Brusgaard K
Laboratory of Developmental Genetics, Academy of Russia, Siberian Department, Novosibirsk, Russia.
Mamm Genome. 1995 Oct;6(10):705-9. doi: 10.1007/BF00354292.
Chromosomal localization of the genes for gamma- and kappa-immunoglobulins (IGGC and IGKC, respectively), aldolase B (ALDB), prion protein (PRNP), homeo box B (HOXB), and glutamate pyruvate transaminase (GPT) were determined with the use of mink-rodent hybrid cells. Analysis of segregation of the mink markers and chromosomes in these hybrid cells allowed us to assign the gene for HOXB to Chromosome (Chr) 8, IGGC to Chr 10, PRNP and IGKC to Chr 11, ALDB to Chr 12, and GPT to Chr 14 in mink. Furthermore, using a set of mink-mouse hybrid cells carrying fragments of mink Chr 8 of different sizes, we assigned the gene for HOXB to the pter-p26 region of the short arm of Chr 8. Comparative mapping of the genes of mink, human, and mouse, as well as other mammalian species, demonstrated that the mink genes HOXB, PRNP, ALDB, and IGGC are members of a conserved region shared by many mammalian species in common; the IGKC gene is a member of a conserved region common to carnivores and primates, not rodents; the GPT gene is a member of a syntenic gene group probably unique to the Mustelidae family or carnivores.
利用水貂-啮齿动物杂交细胞确定了γ-和κ-免疫球蛋白（分别为IGGC和IGKC）、醛缩酶B（ALDB）、朊病毒蛋白（PRNP）、同源盒B（HOXB）和谷丙转氨酶（GPT）基因的染色体定位。对这些杂交细胞中水貂标记和染色体的分离分析使我们能够将HOXB基因定位于水貂的第8号染色体（Chr）、IGGC定位于第10号染色体、PRNP和IGKC定位于第11号染色体、ALDB定位于第12号染色体、GPT定位于第14号染色体。此外，使用一组携带不同大小水貂Chr 8片段的水貂-小鼠杂交细胞，我们将HOXB基因定位于第8号染色体短臂的pter-p26区域。对水貂、人类、小鼠以及其他哺乳动物物种基因的比较图谱分析表明，水貂基因HOXB、PRNP、ALDB和IGGC是许多哺乳动物物种共有的保守区域的成员；IGKC基因是食肉动物和灵长类动物（而非啮齿动物）共有的保守区域的成员；GPT基因是一个可能仅属于鼬科或食肉动物的同线基因组的成员。
