Zhdanova N S, Astakhova N M, Kuznetsov S B, Schuler L, Serov O L
Developmental Genetics Laboratory, Russian Academy of Sciences, Siberian Department, Novosibirsk.
Mamm Genome. 1994 Dec;5(12):781-4. doi: 10.1007/BF00292013.
By fusion of thymidine kinase-deficient mink cells with pig leukocytes, a new type of cell hybrid was produced. It was demonstrated that pig chromosomes segregate in pig-mink hybrids and that hybrid cells contain no cytologically visible rearrangements between the chromosomes of parental species, or chromosome fragmentation. With a set of subclones of two primary hybrid clones, the genes for thymidine kinase-1 (TK1) and uridine 5'-monophosphate hydrolase-2 (UMPH2) were assigned to pig Chromosome (Chr) 12. A cell line with a single pig Chr 8 on the background of mink chromosomes was established. This clone could serve as a source of DNA for building a chromosome-specific library of pig Chr 8. The data obtained suggest that pig-mink cell hybrids can be used for mapping of pig chromosomes.
通过将缺乏胸苷激酶的水貂细胞与猪白细胞融合，产生了一种新型细胞杂种。结果表明，猪染色体在猪 - 水貂杂种中发生分离，并且杂种细胞在亲本物种的染色体之间没有细胞学上可见的重排或染色体片段化。利用两个原代杂种克隆的一组亚克隆，将胸苷激酶 -1（TK1）和尿苷5'-单磷酸水解酶 -2（UMPH2）基因定位到猪12号染色体（Chr）上。在水貂染色体背景上建立了一个带有单条猪8号染色体的细胞系。该克隆可作为构建猪8号染色体特异性文库的DNA来源。所获得的数据表明，猪 - 水貂细胞杂种可用于猪染色体的图谱绘制。
