Liu Q, Ohshima K, Shinohara T, Kikuchi M
First Department of Pathology, School of Medicine, Fukuoka University, Japan.
Pathol Int. 1995 Oct;45(10):729-34. doi: 10.1111/j.1440-1827.1995.tb03389.x.
Cell death can now be divided into necrosis and apoptosis, which are different in their morphology, biochemistry and biological significance. The present study was designed to investigate cell death in histiocytic necrotizing lymphadenitis (HNL). The features of cell death in 10 cases of HNL were analyzed using histiomorphology, ultrastructure and in situ apoptosis detection (ApopTag) methods. Two patterns of cell death were discerned. One was apoptosis of individual cells and the other was necrosis. The first pattern could be observed in all cases and the morphological features of the dead cells were consistent with those of apoptosis, which included distinctive cell volume shrinking and chromatin condensation. The apoptotic cells and bodies could frequently be found to be phagocytosed by the histiocytes. ApopTag was positively stained in most of the morphologically apoptotic cells. By double staining, most ApopTag positive cells were found to be T lymphocytes. A previous report showed that the majority of the proliferative cells were T lymphocytes. Based on those results, if was speculated that the main pathological characteristics of HNL therefore consisted of apoptosis and the proliferation of T lymphocytes.
细胞死亡现在可分为坏死和凋亡，二者在形态学、生物化学及生物学意义上均有所不同。本研究旨在探讨组织细胞坏死性淋巴结炎（HNL）中的细胞死亡情况。采用组织形态学、超微结构及原位凋亡检测（ApopTag）方法，分析了10例HNL中细胞死亡的特征。识别出两种细胞死亡模式。一种是单个细胞的凋亡，另一种是坏死。第一种模式在所有病例中均可观察到，死亡细胞的形态学特征与凋亡一致，包括明显的细胞体积缩小和染色质凝聚。凋亡细胞及凋亡小体常被组织细胞吞噬。在大多数形态学上呈凋亡的细胞中，ApopTag呈阳性染色。通过双重染色发现，大多数ApopTag阳性细胞为T淋巴细胞。先前的一份报告显示，大多数增殖细胞为T淋巴细胞。基于这些结果，推测HNL的主要病理特征因此包括凋亡及T淋巴细胞增殖。
