Ishikawa M, Sasajima T, Kubo Y
First Department of Surgery, Asahikawa Medical College, Japan.
Eur J Vasc Endovasc Surg. 1996 Jan;11(1):105-11. doi: 10.1016/s1078-5884(96)80143-2.
To clarify the course of re-endothelialisation (Re-E) in an entire graft and to establish the effect of immersion media for the preservation of endothelial cells.
Autogenous femoral veins of dogs were immersed in heparinised saline solution (n = 18) or heparinized autogenous blood (n = 18). After immersion, the grafts were implanted into bilateral femoral artery, and were retrieved 1 day to 4-8 weeks after implantation.
For the grafts immersed in the heparinised saline solution, the values for % area of endothelial cell coverage before implantation, and at 1 day, 1 week, and 4 weeks after implantation were 44.9%, 6.2%, 14.5%, and 81.3%, respectively. For the grafts immersed in heparinised autogenous blood, the values were 73.5%, 20.6%, 79.2% and 95.5%, respectively. However, such relatively rapid speed of Re-E slowed down considerably after 1 week following implantation in this group.
The use of heparinized autogenous blood is strongly recommended as a preparation media for autogenous vein grafts. Almost all of the endothelial cells fall away in the earlier period after implantation and regenerate multifocally and irregularly. Re-E is incomplete even at 8 weeks after surgery, and we suggest that the area of incomplete Re-E may develop into intimal hyperplasia.
阐明整个移植物中再内皮化（Re-E）的过程，并确定用于保存内皮细胞的浸泡介质的效果。
将犬的自体股静脉浸泡在肝素化盐溶液（n = 18）或肝素化自体血液（n = 18）中。浸泡后，将移植物植入双侧股动脉，并在植入后1天至4 - 8周取出。
对于浸泡在肝素化盐溶液中的移植物，植入前、植入后1天、1周和4周时内皮细胞覆盖面积百分比的值分别为44.9%、6.2%、14.5%和81.3%。对于浸泡在肝素化自体血液中的移植物，这些值分别为73.5%、20.6%、79.2%和95.5%。然而，在该组植入后1周后，这种相对较快的再内皮化速度显著减慢。
强烈推荐使用肝素化自体血液作为自体静脉移植物的制备介质。几乎所有内皮细胞在植入后的早期都会脱落，并多灶性且不规则地再生。即使在手术后8周，再内皮化也不完全，我们认为不完全再内皮化的区域可能会发展为内膜增生。
