Williams G Z, Harris E K, Widdowson G M
Clin Chem. 1977 Jan;23(1):100-4.
Variation in the assays of uniform control serum commonly are assumed to represent day-to-day analytical variation. To test this assumption, we compared the differences between results of serum aliquots assayed immediately for 12 constituents and frozen aliquots accumulated and assayed on a single day with the results of control serum variation from the same period. One aliquot of each weekly sample was stored frozen. Eleven subjects were sampled for 12 weeks. Storage at --20 degrees C for 15 weeks had a mild destructive effect on two enzymes in serum. The control serum data revealed significant linear trends in magnesium (upwards) and alkaline phosphatase (downwards) that substantially increased the respective variances. In the other 10 constituents tested, comparison of variances indicated that long-term (weeks) variation in control serum assays is similar to the difference of variation between aliquots assayed immediately and those frozen and assayed at the same time. For these constituents, this finding justifies the use of control serum to estimate long term analytical variation.
通常认为统一对照血清检测结果的差异代表日常分析差异。为验证这一假设，我们比较了12种成分即时检测的血清等分样本结果与积累后在同一天检测的冷冻等分样本结果之间的差异，以及同一时期对照血清差异的结果。每周样本的一份等分样本冷冻保存。对11名受试者进行了12周的采样。在-20℃下储存15周对血清中的两种酶有轻微的破坏作用。对照血清数据显示镁（上升）和碱性磷酸酶（下降）有显著的线性趋势，这大幅增加了各自的方差。在测试的其他10种成分中，方差比较表明对照血清检测中的长期（数周）差异与即时检测的等分样本和冷冻后同时检测的等分样本之间的差异变化相似。对于这些成分，这一发现证明使用对照血清来估计长期分析差异是合理的。
