Erbing B, Jansson P E, Widmalm G, Nimmich W
Department of Organic Chemistry, Arrhenius Laboratory, Stockholm University, Sweden.
Carbohydr Res. 1995 Aug 25;273(2):197-205. doi: 10.1016/0008-6215(95)00099-f.
The structure of the capsular polysaccharide from the Klebsiella K8 reference strain 1015 has been elucidated. The structure was deduced from sugar analysis, different methylation analyses, a uronic acid degradation, and NMR spectroscopy. It is concluded that the polysaccharide is composed of pentasaccharide repeating units with the structure: [formula: see text] The structure differs from that of the previously published structure of the capsular polysaccharide from Klebsiella K8, which originates from another strain and has the following structure: [formula: see text] The serological similarity between the two strains is most likely derived from a common tetrasaccharide which is substituted in different ways in the two strains. Since the strain in the present investigation originates from the Klebsiella K reference strain collection of the International Escherichia and Klebsiella Centre, Copenhagen, Denmark, it is suggested that it should keep the designation K8. The other polysaccharide with Klebsiella K8 specificity should be renamed as K8,52,59 based on the cross-reactivity of the strain (I. Orskov, unpublished).
已阐明了肺炎克雷伯菌K8参考菌株1015的荚膜多糖结构。该结构是通过糖分析、不同的甲基化分析、糖醛酸降解和核磁共振光谱推导出来的。得出的结论是，该多糖由具有以下结构的五糖重复单元组成：[化学式：见正文] 该结构与先前发表的源自另一菌株的肺炎克雷伯菌K8荚膜多糖结构不同，其结构如下：[化学式：见正文] 这两种菌株之间的血清学相似性很可能源自一种常见的四糖，该四糖在两种菌株中的取代方式不同。由于本研究中的菌株源自丹麦哥本哈根国际大肠杆菌和克雷伯菌中心的肺炎克雷伯菌K参考菌株库，因此建议保留K8的命名。基于该菌株的交叉反应性，另一种具有肺炎克雷伯菌K8特异性的多糖应重新命名为K8,52,59（I. 奥斯科夫，未发表）。
