Keeven J, Wrobel S, Portoles M, DeCicco B T
Microbial Applications Laboratory, Catholic University of America, Washington, DC 20064, USA.
CLAO J. 1995 Oct;21(4):238-41.
We examined the antimicrobial preservative efficacy of nine rigid gas permeable (RGP) contact lens care solutions by challenging them in a laboratory study with both standard test organisms and preservative-resistant strains. Solutions containing polyhexamethylene biguanide (PHMB), such as Boston Advance (15 ppm) and a Boston Advance Enhanced Comfort Formula (5 ppm PHMB + 30 ppm chlorhexidine gluconate [CHG]) were most effective at rapidly killing vegetative cells, killing all bacteria and yeast by 6 hours. Boston Advance only slightly reduced the number of Aspergillus niger spores during the 28 day study, but Boston Advance Enhanced Comfort Formula reduced the spores by almost 3 logs by 14 days and by 4 logs by 21 days. Thimerosal-preserved Soaclens only reduced Staphylococcus aureus by 1 log at 6 hours, but at 1 day all tested organisms including S. aureus and A. niger were killed. Barnes Hind Gas Permeable Wetting and Soaking Solution, containing 50 ppm CHG, had little effect on adapted Serratia marcescens or Pseudomonas cepacia during the 28 day study. Allergan Wet-N-Soak Plus, containing 30 ppm benzalkonium chloride (BAK), was also unable to kill adapted S. marcescens or P. cepacia. CIBA Vision Premus (40 ppm BAK) was unable to kill adapted S. marcescens. Alcon Opti-Free, with 50 ppm polyquaternium-1 (polyquad), did not kill P. cepacia. Sherman Stay-Wet 3, containing 0.1% benzyl alcohol, was not effective against S. marcescens (adapted or unadapted), P. cepacia, or A. niger. Sherman DeStat 3, which contains 0.1% benzyl alcohol plus surfactants and five times the concentration of EDTA as in Stay-Wet 3, was able to kill all the vegetative cells by 7 days but did not significantly reduce A. niger.
我们通过在实验室研究中用标准测试微生物和抗防腐剂菌株对九种硬性透气（RGP）隐形眼镜护理液进行挑战，来检验它们的抗菌防腐剂功效。含有聚六亚甲基双胍（PHMB）的护理液，如Boston Advance（15 ppm）和Boston Advance增强舒适配方（5 ppm PHMB + 30 ppm葡萄糖酸氯己定[CHG]）在快速杀灭营养细胞方面最为有效，6小时内可杀灭所有细菌和酵母菌。在为期28天的研究中，Boston Advance仅略微减少了黑曲霉孢子的数量，但Boston Advance增强舒适配方在14天时使孢子数量减少了近3个对数，在21天时减少了4个对数。含硫柳汞的Soaclens在6小时时仅使金黄色葡萄球菌数量减少了1个对数，但在1天时所有测试微生物包括金黄色葡萄球菌和黑曲霉都被杀死。含有50 ppm CHG的Barnes Hind Gas Permeable Wetting and Soaking Solution在为期28天的研究中对适应性粘质沙雷氏菌或洋葱伯克霍尔德菌几乎没有影响。含有30 ppm苯扎氯铵（BAK）的Allergan Wet-N-Soak Plus也无法杀灭适应性粘质沙雷氏菌或洋葱伯克霍尔德菌。CIBA Vision Premus（40 ppm BAK）无法杀灭适应性粘质沙雷氏菌。含有50 ppm聚季铵盐-1（polyquad）的Alcon Opti-Free不能杀灭洋葱伯克霍尔德菌。含有0.1%苯甲醇的Sherman Stay-Wet 3对粘质沙雷氏菌（适应性或非适应性）、洋葱伯克霍尔德菌或黑曲霉无效。含有0.1%苯甲醇加表面活性剂且乙二胺四乙酸浓度是Stay-Wet 3五倍的Sherman DeStat 3能够在7天内杀灭所有营养细胞，但对黑曲霉没有显著减少作用。
