Sharma S, Ramachandran L, Rao G N
Late Sri Devchand Nagardas Jhaveri Microbiology Centre, Hyderabad, India.
CLAO J. 1995 Oct;21(4):247-51.
Contact lens related Acanthamoeba keratitis has been more often associated with the use of soft contact lenses than with the use of rigid gas permeable (RGP) contact lenses. We studied the adherence of Acanthamoeba cysts and trophozoites to unworn RGP (silicone acrylate and fluoropolymer) and low and high water content soft contact lenses marketed in India. A human corneal isolate of Acanthamoeba polyphaga suspended in saline (1 x 10(5) amoebae/mL) was used. Lens segments were exposed to A. polyphaga cysts or trophozoites with or without shaking. After exposure for zero to 7 hours, lenses were either washed or not washed and the adherence was determined using light microscopy and a hemocytometer (per square millimeter of the lens). Both cysts and trophozoites immediately adhered to all four types of lenses. Adhesion increased with an increase in exposure time. The number of adherent cysts and trophozoites was higher (P = 0.043-0.0003) in the unwashed group of lenses at all exposure times for all lenses. Among washed groups, adherence was greatest with RGP silicone acrylate and high water content soft lenses. Trophozoites of Acanthamoeba showed greater adherence to RGP lenses, whereas cysts showed greater adherence to high water content soft lenses. Shaking did not have a significant effect on adherence. Adhesion of Acanthamoeba to contact lenses may play an important role in the pathogenesis of Acanthamoeba keratitis.
与隐形眼镜相关的棘阿米巴角膜炎与使用软性隐形眼镜的关联比与使用硬性透气性（RGP）隐形眼镜的关联更为常见。我们研究了棘阿米巴囊肿和滋养体对印度市场上销售的未使用过的RGP（硅丙烯酸酯和含氟聚合物）以及低含水量和高含水量软性隐形眼镜的黏附情况。使用了悬浮在盐水中（1×10⁵个变形虫/毫升）的多噬棘阿米巴人角膜分离株。将镜片片段暴露于多噬棘阿米巴囊肿或滋养体中，有或没有摇晃。暴露0至7小时后，对镜片进行或不进行冲洗，然后使用光学显微镜和血细胞计数器（每平方毫米镜片）确定黏附情况。囊肿和滋养体均立即黏附于所有四种类型的镜片。黏附随着暴露时间的增加而增加。在所有暴露时间下，所有镜片未冲洗组中黏附的囊肿和滋养体数量更高（P = 0.043 - 0.0003）。在冲洗组中，RGP硅丙烯酸酯和高含水量软性镜片的黏附性最强。棘阿米巴滋养体对RGP镜片的黏附性更强，而囊肿对高含水量软性镜片的黏附性更强。摇晃对黏附没有显著影响。棘阿米巴对隐形眼镜的黏附可能在棘阿米巴角膜炎的发病机制中起重要作用。
