Department of Ophthalmology and Visual Sciences, Paulista School of Medicine, São Paulo Hospital, Federal University of São Paulo, São Paulo, SP, Brazil - UNIFESP.
Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil - UFRGS.
Invest Ophthalmol Vis Sci. 2024 May 1;65(5):4. doi: 10.1167/iovs.65.5.4.
To investigate the adhesion of Acanthamoeba to scleral contact lens (ScCL) surface according to lens shape.
Two strains of A. polyphaga (CDC:V062 and ATCC 30461) and one clinical Acanthamoeba isolate, were inoculated onto five contact lens (CL): one first-generation silicone hydrogel (SHCL; lotrafilcon B; adhesion control) containing plasma surface treatment; two ScCL (fluorosilicone acrylate) one containing surface treatment composed of plasma and the other containing plasma with Hydra-PEG, and two CL designed with a flat shape having the same material and surface treatments of the ScCL. Trophozoites that adhered to the lens's surfaces were counted by inverted optical light microscopy. Possible alterations of the lens surface that could predispose amoeba adhesion and Acanthamoeba attached to these lens surfaces were evaluated by scanning electron microscopy (SEM).
All strains revealed greater adhesion to the ScCL when compared with the flat lenses (P < 0.001). The clinical isolate and the ATCC 30461 had a higher adhesion (P < 0.001) when compared with the CDC:V062. A rough texture was observed on the surface of the lenses that have been examined by SEM. Also, SEM revealed that the isolates had a rounded appearance on the surface of the ScCL in contrast with an elongated appearance on the surface of the silicone hydrogel.
The findings revealed that the curved shape of the ScCL favors amoeba adhesion.
根据镜片形状研究棘阿米巴对巩膜接触镜（ScCL）表面的黏附。
将两种棘阿米巴属多形虫（CDC：V062 和 ATCC 30461）和一株临床棘阿米巴分离株接种到五种接触镜（CL）上：一种第一代硅水凝胶（SHCL；lotrafilcon B；黏附对照），含有等离子体表面处理；两种 ScCL（氟硅丙烯酸盐），一种含有等离子体和另一种含有等离子体与 Hydra-PEG 的表面处理，以及两种设计为具有相同材料和平坦形状的 ScCL。倒置光学显微镜计数黏附在镜片表面的滋养体。扫描电子显微镜（SEM）评估可能改变镜片表面，使阿米巴易于黏附的因素，以及黏附在这些镜片表面上的棘阿米巴。
与平坦镜片相比，所有菌株对 ScCL 的黏附性均更高（P<0.001）。与 CDC：V062 相比，临床分离株和 ATCC 30461 的黏附性更高（P<0.001）。SEM 观察到，经过检查的镜片表面具有粗糙纹理。此外，SEM 显示，与在硅水凝胶表面的细长外观相比，分离株在 ScCL 表面具有圆形外观。
研究结果表明，ScCL 的弯曲形状有利于阿米巴黏附。
