荧光原位杂交和引物原位标记法，一种快速染色体筛查策略：应用于人类精子非整倍体评估
FISH and PRINS, a strategy for rapid chromosome screening: application to the assessment of aneuploidy in human sperm.
作者信息
Pellestor F, Quenesson I, Coignet L, Girardet A, Andréo B, Lefort G, Charlieu J P
机构信息
CNRS UPR 9008, St. Charles Hospital, Montpellier, France.
出版信息
Cytogenet Cell Genet. 1996;72(1):34-6. doi: 10.1159/000134155.
PMID:8565628
Abstract
The co-utilization of FISH and PRINS techniques for in situ chromosome screening was tested on human sperm nuclei. We used a centromeric repeat probe specific for chromosome 4 for FISH. PRINS reactions were performed with alpha-satellite primers specific for either chromosomes 9 or chromosome 18. Double labeling was obtained and estimates of disomy rates were carried out for the three chromosomes.
摘要
我们在人类精子核上测试了荧光原位杂交（FISH）和引物原位标记（PRINS）技术联合用于原位染色体筛查的效果。我们使用针对4号染色体的着丝粒重复探针进行FISH。PRINS反应则使用针对9号或18号染色体的α-卫星引物进行。实现了双重标记，并对这三条染色体的二体率进行了估计。
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