Bertuzzi F, Zacchetti D, Berra C, Socci C, Pozza G, Pontiroli A E, Grohovaz F
Medicine Department, DIBIT, San Raffaele Scientific Inst., Milano, Italy.
FEBS Lett. 1996 Jan 22;379(1):21-5. doi: 10.1016/0014-5793(95)01422-5.
Insulin release was investigated in parallel with changes in cytosolic calcium concentration, [Ca2+]i, in pig islets stimulated by glucose. After two days in culture, glucose stimulation failed to induce insulin release, and caused limited [Ca2+]i changes in few cells. After ten days, insulin response was partially restored and [Ca2+]i recordings revealed a slow oscillatory activity of the whole islet. Slow oscillations appeared to be due to the average [Ca2+]i variations resulting from the spreading of waves throughout the islet. These waves demonstrate the reestablishment of functional cell coupling, which appears to play a critical role in insulin release.
在葡萄糖刺激猪胰岛的过程中，对胰岛素释放与胞质钙浓度（[Ca2+]i）的变化进行了平行研究。培养两天后，葡萄糖刺激未能诱导胰岛素释放，且仅在少数细胞中引起有限的[Ca2+]i变化。培养十天后，胰岛素反应部分恢复，[Ca2+]i记录显示整个胰岛存在缓慢的振荡活动。缓慢振荡似乎是由波在整个胰岛中传播导致的平均[Ca2+]i变化引起的。这些波表明功能性细胞耦合的重新建立，这似乎在胰岛素释放中起关键作用。
