Rentsch J, Chiesi M
Research Department Ciba-Geigy Ltd., Basel, Switzerland.
FEBS Lett. 1996 Jan 22;379(1):55-9. doi: 10.1016/0014-5793(95)01485-3.
mRNA levels of the ob gene product, leptin, were investigated by quantitative competitive RT-PCR in a mouse cell line (3T3-L1) which can be induced to differentiate into adipocytes. During conversion to fat cells, the level of leptin mRNA increased several-fold and in parallel to that for typical adipocyte markers like lipoprotein lipase, adipsin and glycerophosphate dehydrogenase. Leptin transcription, however, did not correlate with the size of the adipocytes measured as total triglycerides. On the other hand, mRNA levels for leptin in fully differentiated adipocytes were increased 2-3 fold by insulin. In contrast, free fatty acids exerted a concentration-dependent inhibition of leptin transcription while the corticosteroid dexamethasone and an elevation of intracellular cAMP displayed only marginal inhibitory effects on leptin mRNA levels.
通过定量竞争性逆转录聚合酶链反应（RT-PCR），在可诱导分化为脂肪细胞的小鼠细胞系（3T3-L1）中研究了肥胖基因（ob基因）产物瘦素的mRNA水平。在向脂肪细胞转化的过程中，瘦素mRNA水平增加了数倍，且与典型的脂肪细胞标志物如脂蛋白脂肪酶、脂肪酶和甘油磷酸脱氢酶的水平平行。然而，瘦素转录与以总甘油三酯衡量的脂肪细胞大小无关。另一方面，胰岛素使完全分化的脂肪细胞中瘦素的mRNA水平增加2至3倍。相比之下，游离脂肪酸对瘦素转录具有浓度依赖性抑制作用，而皮质类固醇地塞米松和细胞内cAMP升高对瘦素mRNA水平仅显示出轻微的抑制作用。
