Graham M F, Willey A, Adams J, Yager D, Diegelmann R F
Department of Pediatrics and Surgery, Medical College of Virginia, Virginia Commonwealth University, Richmond, USA.
Gastroenterology. 1996 Feb;110(2):344-50. doi: 10.1053/gast.1996.v110.pm8566579.
BACKGROUND & AIMS: Smooth muscle cells resident in the intestinal wall play a significant role in the healing of the injured intestine and in the fibrosis that complicates Crohn's disease. The cytokine interleukin 1 beta (IL-1 beta) is involved in inflammatory bowel disease. The aim of this study was to determine the action of IL-1 beta on proliferation and collagen metabolism in human intestinal smooth muscle cells.
IL-beta caused a three-fold increase in [3H]thymidine uptake at 100 pmol/L. This mitogenic effect was equipotent with that of platelet-derived growth factor when cells were exposed to IL-beta for 48 vs. 24 hours. IL-beta inhibited the secretion of procollagen into culture medium by 70% and the accumulation of newly synthesized procollagen in cells by 55%. In addition, IL-beta caused a concentration-dependent inhibition of steady-state levels of procollagen I and III messenger RNA (85% inhibition at 100 pmol/L) and a 3-5-fold augmentation of collagenase messenger RNA levels.
IL-beta is mitogenic for human intestinal smooth muscle cells, but this action is associated with a concomitant down-regulation of collagen synthesis and secretion and an augmention of collagenase expression.
肠壁中的平滑肌细胞在受损肠道的愈合以及克罗恩病并发的纤维化过程中发挥着重要作用。细胞因子白细胞介素1β（IL-1β）与炎症性肠病有关。本研究的目的是确定IL-1β对人肠道平滑肌细胞增殖和胶原代谢的作用。
100 pmol/L的IL-β使[3H]胸腺嘧啶核苷摄取量增加了三倍。当细胞分别暴露于IL-β 48小时和24小时时，这种促有丝分裂作用与血小板衍生生长因子的作用相当。IL-β使培养基中前胶原的分泌减少70%，细胞中新合成前胶原的积累减少55%。此外，IL-β导致前胶原I和III信使核糖核酸稳态水平呈浓度依赖性抑制（100 pmol/L时抑制85%），胶原酶信使核糖核酸水平增加3至5倍。
IL-β对人肠道平滑肌细胞有促有丝分裂作用，但这种作用伴随着胶原合成和分泌的下调以及胶原酶表达的增加。
