Zuo J, Curtis L M, Yao X, ten Cate W J, Bagger-Sjöbäck D, Hultcrantz M, Rarey K E
Department of Anatomy, Cell Biology, College of Medicine, University of Florida, Gainesville 32610, USA.
Hear Res. 1995 Jul;87(1-2):220-7. doi: 10.1016/0378-5955(95)00092-i.
The glucocorticoid receptor (GR) expression in the neonatal rat cochlea was investigated by utilization of a polyclonal antibody against GR, the immunoreactivity of which exhibited a distinct, age-dependent developmental pattern in tissues of the spiral ligament (SL). Immunostaining of GR appeared initially at the 7th postnatal day (PND), increased rapidly between the 14th and 21st PND, and reached adult-like expression levels by the 21st PND. Less pronounced, developmentally regulated expression patterns of GR were observed in cells of the spiral limbus (SLi), spiral ganglion (SG), organ of Corti (OC), and cochlear nerve (CN). For example, high expression levels of GR were observed in the SLi, SG and OC at 3 PND; subsequently, GR immunoreactivity levels decreased from 7 to 14 PND, and then GR immunoreactivity intensified in these regions by 21 PND. No remarkable changes in GR expression were observed in stria vascularis (SV). These data indicate that GR expression in the inner ear is tissue and age-specific, and that GR expression parallels both Na,K-ATPase expression and endocochlear potential development.
利用抗糖皮质激素受体（GR）的多克隆抗体，研究新生大鼠耳蜗中GR的表达情况。该抗体的免疫反应性在螺旋韧带（SL）组织中呈现出明显的、依赖年龄的发育模式。GR的免疫染色最初出现在出生后第7天（PND），在第14天至第21天之间迅速增加，并在第21天达到类似成年动物的表达水平。在螺旋缘（SLi）、螺旋神经节（SG）、柯蒂氏器（OC）和耳蜗神经（CN）的细胞中，观察到GR的表达模式发育调控不太明显。例如，在出生后第3天，在SLi、SG和OC中观察到GR的高表达水平；随后，GR免疫反应性水平在第7天至第14天下降，然后在这些区域中，到第21天GR免疫反应性增强。在血管纹（SV）中未观察到GR表达的显著变化。这些数据表明，内耳中GR的表达具有组织和年龄特异性，并且GR的表达与钠钾ATP酶的表达以及内淋巴电位的发育平行。
