Zuo J, Rarey K E
Department of Anatomy & Cell Biology, College of Medicine, University of Florida, Gainesville 32610, USA.
Acta Otolaryngol. 1996 May;116(3):422-8. doi: 10.3109/00016489609137867.
The effects of thyroid hormone on Na, K-ATPase subunit isoforms under euthyroid (EUTH), hypothyroid (HYPO) and hyperthyroid (HYPER) states were investigated via immunocytochemistry and the use of polyclonal antibodies specific to each isoform (alpha 1, alpha 2, alpha 3 and beta 1, beta 2). In HYPO animals, there was a distinct decrease in Na, K-ATPase alpha 1 isoform immunoreactivity in the stria vascularis (SV), spiral ganglion (SG) cells, spiral limbus (SLi) and cochlear nerve (CN) as compared with that in EUTH animals by the 17th day of the experiment. Immunostaining of the alpha 1 isoform increased in HYPER animals as compared with that in HYPO animals, and reached a level comparable to that in EUTH animals after 2 days of triiodothyronine (T3) treatment. Levels of alpha 2, alpha 3 and beta 2 isoforms did not appear to be affected by T3 administration. By the 19th day of a low I2 diet, the immunoreactive intensity of the beta 1 isoform was reduced in cochlear tissues of HYPO animals as compared with that in EUTH animals. The immunoreactivity of the beta 1 isoform increased after treatment with T3 for 4 days and was comparable with levels in EUTH animals. These data indicate that alpha 1 and beta 1 isoforms within specific cochlear regions of the adult rat are responsive to thyroid hormone.
通过免疫细胞化学方法以及使用针对每种亚型（α1、α2、α3和β1、β2）的多克隆抗体，研究了甲状腺激素在正常甲状腺（EUTH）、甲状腺功能减退（HYPO）和甲状腺功能亢进（HYPER）状态下对钠钾ATP酶亚基亚型的影响。在实验第17天，与EUTH动物相比，HYPO动物的血管纹（SV）、螺旋神经节（SG）细胞、螺旋缘（SLi）和蜗神经（CN）中钠钾ATP酶α1亚型的免疫反应性明显降低。与HYPO动物相比，HYPER动物中α1亚型的免疫染色增加，在三碘甲状腺原氨酸（T3）治疗2天后达到与EUTH动物相当的水平。α2、α3和β2亚型的水平似乎不受T3给药的影响。在低碘饮食第19天，与EUTH动物相比，HYPO动物耳蜗组织中β1亚型的免疫反应强度降低。用T3治疗4天后，β1亚型的免疫反应性增加，与EUTH动物的水平相当。这些数据表明，成年大鼠特定耳蜗区域内的α1和β1亚型对甲状腺激素有反应。
