Focarelli R, Giuffrida A, Rosati F
Department of Evolutionary Biology, University of Siena, Italy.
Hum Reprod. 1995 Oct;10(10):2755-9. doi: 10.1093/oxfordjournals.humrep.a135787.
Changes in the distribution of sialylglycoconjugates on the surface of uncapacitated and in-vitro capacitated human spermatozoa were studied by means of two sialic acid specific lectins (Maackia amurensis agglutinin and Sambucus nigra agglutinin). On the uncapacitated sperm surface, sialylglycoconjugates were found to be localized from the post-acrosomal region of the sperm head to the tail middle piece, whereas after in-vitro capacitation these molecules were only found in a small area of the post-acrosomal region. The surface of capacitated human spermatozoa was also investigated by specifically radiolabelling its terminal sialic acid residues. A 20 kDa glycoprotein, which was partially purified by anion-exchange chromatography, was the main component of the sialylglycoconjugate pattern after in-vitro capacitation.
利用两种唾液酸特异性凝集素（黑果腺肋花楸凝集素和接骨木凝集素）研究了未获能和体外获能的人类精子表面唾液酸糖缀合物分布的变化。在未获能的精子表面，发现唾液酸糖缀合物从精子头部顶体后区域定位到尾部中段，而体外获能后，这些分子仅在顶体后区域的一小部分被发现。还通过特异性放射性标记其末端唾液酸残基来研究获能人类精子的表面。一种通过阴离子交换色谱法部分纯化的20 kDa糖蛋白是体外获能后唾液酸糖缀合物模式的主要成分。
