Ueda T, Kajita K, Fujimori O
Second Department of Anatomy, Nagoya City University Medical School, Nagoya, Japan.
Histochem J. 1998 Sep;30(9):667-76. doi: 10.1023/a:1003400307405.
A variety of sialic acids contained in the rat epididymis during post-natal development were examined by means of lectin and carbohydrate histochemistry. Epididymides from male Sprague-Dawley rats on post-natal days 14, 21, 30, 39, 49, 56 and 70 were fixed in Bouin's fluid and embedded routinely in paraffin wax. Hydrated sections were subjected either to the lectin methods using biotinylated Sambucus sieboldiana lectin or Maackia amurensis lectin or to the selective periodate oxidation-phenylhydrazine-thiocarbohydrazide-silver protein-physical development technique with or without saponification. The results revealed that sialic acids appeared in the epididymal epithelium at day 14, followed by particular distribution patterns corresponding to cell differentiation during days 21-39. High-level O-acetylation of sialic acids was observed in the principal cells of the initial segment and proximal caput after day 39. These results suggest that sialic acids with different linkages and O-acetylation become adult in distribution at the 'differentiation' period under the influence of androgen, before spermatozoa reach the epididymal lumen. Such carbohydrates may be correlated, at least in part, with sperm-binding sialoproteins, which increase dramatically during the window between days 21 and 39.
采用凝集素和碳水化合物组织化学方法，对出生后发育过程中大鼠附睾内所含的多种唾液酸进行了检测。取出生后第14、21、30、39、49、56和70天的雄性斯普拉格-道利大鼠的附睾，用布安氏液固定，常规石蜡包埋。水化切片分别采用生物素化的黑接骨木凝集素或山槐凝集素的凝集素方法，或采用选择性高碘酸盐氧化-苯肼-硫代碳酰肼-银蛋白-物理显影技术（有无皂化处理）。结果显示，唾液酸在第14天出现在附睾上皮中，随后在第21至39天呈现出与细胞分化相对应的特定分布模式。在第39天后，在起始段和近端头部的主细胞中观察到唾液酸的高水平O-乙酰化。这些结果表明，在精子到达附睾管腔之前，在雄激素的影响下，具有不同连接方式和O-乙酰化的唾液酸在“分化”期达到成年期分布。这些碳水化合物可能至少部分与精子结合唾液蛋白相关，后者在第21天至39天之间显著增加。
