Chan S, Henderson W R, Li S H, Hanifin J M
Oregon Health Sciences University, Department of Dermatology, Portland 97201-3098, USA.
J Allergy Clin Immunol. 1996 Jan;97(1 Pt 1):85-94. doi: 10.1016/s0091-6749(96)70286-5.
Past studies of peripheral blood mononuclear cells (PBMC) from patients with atopic dermatitis (AD) have demonstrated reduced proliferation. We have studied phytohemagglutinin-induced lymphocyte proliferation in the context of interleukin-4 (IL-4), interferon-gamma (IFN-gamma), and prostaglandin E2 (PGE2) production in cultures of PBMC from patients without and with AD. The proliferation index was found to correlate proportionally to IFN-gamma production and inversely to T-cell IL-4 and monocyte PGE2 production. Assays in parallel cultures showed significantly increased PGE2 production by purified AD monocytes. The proliferation index in PBMC from persons with AD was significantly reduced compared with normal PBMC. This difference was normalized in the presence of extrinsic IFN-gamma but exaggerated when IL-4 was added. Increased AD monocyte production of inflammatory factors (e.g., PGE2) and cytokines appears to increase IL-4 production by Th2 while suppressing IFN-gamma production by Th1. Restoration of the normal proliferation of PBMC by the addition of IFN-gamma may represent one mechanism for the clinical efficacy of IFN-gamma treatment of AD.
既往对特应性皮炎（AD）患者外周血单个核细胞（PBMC）的研究显示其增殖能力降低。我们研究了在无AD患者和AD患者的PBMC培养物中，植物血凝素诱导的淋巴细胞增殖情况，以及白细胞介素-4（IL-4）、干扰素-γ（IFN-γ）和前列腺素E2（PGE2）的产生。结果发现增殖指数与IFN-γ的产生成比例相关，与T细胞IL-4和单核细胞PGE2的产生呈负相关。平行培养实验显示，纯化的AD单核细胞产生的PGE2显著增加。与正常PBMC相比，AD患者PBMC的增殖指数显著降低。在存在外源性IFN-γ时，这种差异恢复正常，但添加IL-4时差异会扩大。AD单核细胞产生的炎症因子（如PGE2）和细胞因子增加，似乎会增加Th2细胞产生IL-4，同时抑制Th1细胞产生IFN-γ。添加IFN-γ恢复PBMC的正常增殖可能是IFN-γ治疗AD临床疗效的一种机制。
