Reichenbach A, Siegel A, Rickmann M, Wolff J R, Noone D, Robinson S R
Paul Flechsig Institute for Brain Research, Leipzig University, F.R.G.
J Hirnforsch. 1995;36(4):509-17.
We have used immunocytochemistry for glial fibrillary adidic protein and glutamine synthetase to selectively label Bergmann glia in the adult rat cerebellum. From measurements of radial, tangential and en face sections we provide new data on the distribution and disposition of these glial cells. Specifically, Bergmann glia were found to have a mean areal packing density of 8,269 somata/mm2, their radial processes are packed at a mean density of 39,000/mm2, and their endfeet at the pial surface have a mean density of 19,973/mm2. Each Bergmann glial cell ist "responsible" for the equivalent of a column of cerebellar cortex having a base of 11 microns x 11 microns, a height of 170 microns, and a volume of 20,559 microns3. There are 8.1 Bergmann glia for each Purkinje cell, and each glial cell ensheaths between 2,142 and 6,358 Purkinje cell synapses. We use these data to offer insights on the roles of Bergmann glia during development and in the adult brain.
我们运用免疫细胞化学方法，通过检测胶质纤维酸性蛋白和谷氨酰胺合成酶，对成年大鼠小脑的伯格曼胶质细胞进行选择性标记。通过对径向、切向和正面切片的测量，我们提供了关于这些胶质细胞分布和排列的新数据。具体而言，发现伯格曼胶质细胞的平均面密度为8269个细胞体/mm²，其径向突起的平均密度为39000/mm²，其在软脑膜表面的终足平均密度为19973/mm²。每个伯格曼胶质细胞“负责”相当于一个小脑皮质柱，其底部为11微米×11微米，高度为170微米，体积为20559立方微米。每个浦肯野细胞对应8.1个伯格曼胶质细胞，每个胶质细胞包裹2142至6358个浦肯野细胞突触。我们利用这些数据来深入了解伯格曼胶质细胞在发育过程和成年大脑中的作用。
