Engels W, Reiters P H, Daemen M J, Smits J F, van der Vusse G J
Cardiovascular Research Institute Maastricht, University of Limburg, The Netherlands.
J Pathol. 1995 Dec;177(4):423-9. doi: 10.1002/path.1711770414.
The cardiac distribution of mast cells was investigated after the induction of acute myocardial infarction in the rat. The left anterior descending coronary artery (LAD) was occluded by ligation in the infarct group, whereas in sham rats only a superficial ligature was placed beside the LAD. Rats of both groups were killed at 4, 7, 14, 21, 35, and 85 days following surgery. Hearts were excised and formalin-fixed. Mast cell densities were monitored in subepicardial and subendocardial layers of the left ventricle (LV) in 6 microns thick toluidine blue-stained cross-sections. In control (non-operated) animals, mast cell densities were comparable in the LV subepicardial and subendocardial layers (1.5-2.0 cells per mm2). Following infarction, the mast cell density at the subepicardial site of the infarction gradually increased, reaching a maximum of 25 cells per mm2 on day 21, while a non-significant increase was observed at the subendocardial site. In the non-infarcted regions, the mast cell density increased transiently to reach a maximum of 7 cells per mm2 on day 35 in the subepicardial layer. Again, changes in mast cell density in the subendocardial layer were non-significant. In the sham group, a gradual increase to 9 cells per mm2 on day 21 and a subsequent decrease to 5 cells per mm2 on day 85 were observed in the subepicardial layers. These findings indicate a massive accumulation of mast cells in the subepicardial layers of the infarcted region and a small but significant effect of the surgical procedure on cardiac mast cell deposition, especially in the outer layers of the left ventricle.
在大鼠急性心肌梗死诱导后，研究了肥大细胞的心脏分布情况。梗死组通过结扎闭塞左冠状动脉前降支（LAD），而假手术大鼠仅在LAD旁进行浅结扎。两组大鼠均在术后4、7、14、21、35和85天处死。取出心脏并用福尔马林固定。在6微米厚的甲苯胺蓝染色横切面上，监测左心室（LV）心外膜下和心内膜下层的肥大细胞密度。在对照（未手术）动物中，LV心外膜下和心内膜下层的肥大细胞密度相当（每平方毫米1.5 - 2.0个细胞）。梗死发生后，梗死灶心外膜部位的肥大细胞密度逐渐增加，在第21天达到每平方毫米25个细胞的最大值，而在心内膜部位观察到的增加不显著。在非梗死区域，心外膜层的肥大细胞密度在第35天短暂增加至每平方毫米7个细胞的最大值。同样，心内膜层肥大细胞密度的变化不显著。在假手术组中，心外膜层在第21天逐渐增加至每平方毫米9个细胞，随后在第85天降至每平方毫米5个细胞。这些发现表明梗死区域的心外膜层有大量肥大细胞积聚，并且手术操作对心脏肥大细胞沉积有微小但显著的影响，尤其是在左心室外层。
