Waberski D, Südhoff H, Hahn T, Jungblut P W, Kallweit E, Calvete J J, Ensslin M, Hoppen H O, Wintergalen N, Weitze K F
Institute for Reproductive Medicine, Veterinary School of Hannover, Germany.
J Reprod Fertil. 1995 Nov;105(2):247-52. doi: 10.1530/jrf.0.1050247.
The shortening of the time interval between the onset of oestrus and ovulation in sows by the transcervical administration of seminal plasma was investigated in 23 German Landrace gilts, using the technique of single horn infusions (Mariensee model) in combination with the transcutaneous sonographic monitoring of ovaries. Preparative surgery comprised the detachment of the left uterine horn from the corpus, leaving the caudal end open to the peritoneal cavity but sealing the corpus wound. The left ovary was loosely tied to the ventral abdominal wall for better sonographic distinction. The animals were used in two to four consecutive cycles. After detection of oestrus by the teaser boar, the patent (right) horns were filled by transcervical infusion of 100 ml of a variety of test solutions. Ovulation was probed by transcutaneous sonography at intervals of 4 h thereafter. Native seminal plasma provoked ovulation in the ipsilateral ovary of the treated horn 10.7 h earlier than in the contralateral ovary. This effect was reduced to 7.3 h after charcoal treatment of seminal plasma; addition of 10 micrograms oestradiol restored the effect in full, while 10 micrograms of oestradiol in PBS shortened the time interval to only 3.3 h versus the control ovary. Little effect was seen with oestrone sulfate, none with prostaglandins in PBS or with PBS alone. The preliminary characterization of the nonsteroidal component of seminal plasma advancing ipsilateral ovulation after transcervical infusion suggests a proteinaceous nature. The activity resides in the 1-10 kDa fraction separated by ultrafiltration and is lost after treatment with pronase.
在23头德国长白母猪中，采用单角灌注技术（Mariensee模型）并结合经皮超声监测卵巢，研究经子宫颈给予精浆对母猪发情开始至排卵时间间隔的缩短作用。术前手术包括将左子宫角从子宫体分离，使尾端向腹腔开放，但封闭子宫体伤口。将左卵巢松散地系于腹前壁，以便更好地进行超声区分。这些动物连续使用两到四个周期。用试情公猪检测到发情后，经子宫颈向通畅的（右侧）子宫角灌注100 ml各种测试溶液。此后每隔4小时通过经皮超声检查探测排卵情况。天然精浆使处理侧子宫角同侧卵巢的排卵时间比未处理侧卵巢提前10.7小时。精浆经活性炭处理后，这种作用降至7.3小时；添加10微克雌二醇可完全恢复该作用，而在PBS中添加10微克雌二醇使与对照卵巢相比的时间间隔仅缩短至3.3小时。硫酸雌酮几乎没有作用，PBS中的前列腺素或单独的PBS均无作用。经子宫颈灌注后促进同侧排卵的精浆非甾体成分的初步特征表明其具有蛋白质性质。该活性存在于通过超滤分离的1 - 10 kDa组分中，用链霉蛋白酶处理后活性丧失。
