Waberski D
Institute for Reproductive Medicine, Veterinary School of Hannover, Germany.
J Reprod Fertil Suppl. 1997;52:105-9.
In pigs, transcervical infusion of seminal plasma at the onset of oestrus advances ovulation and thus improves the chance of gametes meeting during their full fertilizing competence. An animal model that allows single uterine horn infusion was used in combination with transcutaneous sonographic monitoring of ovaries. Preparative surgery involved the detachment of one uterine horn from the corpus, leaving the caudal end open to the peritoneal cavity but sealing the corpus wound. Transcervical infusion of 100 ml seminal plasma immediately after the detection of oestrus advanced ovulation by between 8 and 14 h on the ipsilateral ovary adjacent to the infused horn compared with the contralateral ovary. In addition, the seminal plasma infusion did not influence the LH profile compared with uterine infusion of PBS. This finding indicates that the effect is mediated by a local mechanism in the female genital tract. The advancement of ovulation depends on the time of infusion early in oestrus and is more pronounced in gilts exhibiting a long interval between the onset of oestrus and spontaneous ovulation compared with early ovulators. At 24 h after the detection of oestrus, seminal plasma was ineffective. Apparently, seminal plasma does not affect maturation and fertilizing competence of oocytes. The activity resides in a low molecular mass protein fraction and, to a far lesser extent, in seminal oestrogens. Further characterization of the active components may allow a practical application in AI.
在猪身上，发情开始时经宫颈注入精浆可使排卵提前，从而提高配子在完全具备受精能力期间相遇的几率。使用了一种允许对单个子宫角进行注入的动物模型，并结合经皮超声监测卵巢。准备手术包括将一个子宫角从子宫体分离，使尾端向腹腔开放，但封闭子宫体伤口。与对侧卵巢相比，在检测到发情后立即经宫颈注入100毫升精浆可使与注入角相邻的同侧卵巢的排卵提前8至14小时。此外，与注入PBS的子宫相比，精浆注入对促黄体生成素（LH）曲线没有影响。这一发现表明，该效应是由女性生殖道中的局部机制介导的。排卵提前取决于发情早期的注入时间，并且与早期排卵的后备母猪相比，发情开始与自然排卵之间间隔时间长的后备母猪中这种效应更明显。在检测到发情后24小时，精浆无效。显然，精浆不会影响卵母细胞的成熟和受精能力。该活性存在于低分子量蛋白质部分，在精浆雌激素中的程度要小得多。对活性成分的进一步表征可能会使其在人工授精中得到实际应用。
