Waberski D, Kremer H, Borchardt Neto G, Jungblut P W, Kallweit E, Weitze K F
Institute for Reproductive Medicine, School of Veterinary Medicine Hannover, Germany.
Zentralbl Veterinarmed A. 1999 Sep;46(7):431-8. doi: 10.1046/j.1439-0442.1999.00230.x.
Previous studies showed that intrauterine infusion of seminal plasma at the onset of oestrus could advance ovulation in pigs, possibly to enhance the chances of fertilization by optimizing the chronological events of fertilization. This effect has been attributed to a local unilateral mechanism whereby infusion into a single uterine horn advances ovulation in the adjacent ovary. The present study was designed to elucidate possible mechanisms of local signal transduction. In a series of five experiments using 43 gilts, the ovarian response was investigated after infusion of seminal plasma at different sites of the female reproductive tract. The time of ovulation was detected sonographically at 4- or 2-h intervals. Single uterine horn infusion of 100 ml seminal plasma advanced ovulation on the ipsilateral ovary by 9.3 h (mean) compared with the contralateral ovary. Dissection of the ipsilateral isthmus abolished the unilateral seminal plasma effect. Unilateral infusion of 50 microliters or 1 ml seminal plasma or 50 microliters of the concentrated 1-10 kDa fraction in the lower isthmus was ineffective. Application of 5 ml seminal plasma into the tip of a ligated uterine horn lead to 3.6 h (mean) earlier ovulation on the adjacent ovary. In contrast, the infusion of 5 ml NaCl showed no effect. Application of 5 ml seminal plasma in the middle of the uterine horn between two ligatures was ineffective. It is concluded that, for the transduction of the local signal involved in the advancement of ovulation, contact of seminal plasma with the epithelium of the utero-tubal junction is essential.
先前的研究表明，在发情开始时向子宫内注入精浆可使猪的排卵提前，这可能是通过优化受精的时间顺序来增加受精几率。这种效应归因于一种局部单侧机制，即向单个子宫角注入精浆会使相邻卵巢的排卵提前。本研究旨在阐明局部信号转导的可能机制。在一系列五项实验中，对43头后备母猪进行了研究，在雌性生殖道的不同部位注入精浆后，观察卵巢反应。每隔4小时或2小时通过超声检查检测排卵时间。与对侧卵巢相比，向单个子宫角注入100毫升精浆可使同侧卵巢的排卵提前9.3小时（平均）。解剖同侧峡部可消除精浆的单侧效应。在下峡部单侧注入50微升或1毫升精浆或50微升浓缩的1 - 10 kDa组分无效。向结扎子宫角的尖端注入5毫升精浆可使相邻卵巢的排卵提前3.6小时（平均）。相比之下，注入5毫升氯化钠没有效果。在两个结扎之间的子宫角中部注入5毫升精浆无效。得出的结论是，对于排卵提前所涉及的局部信号转导，精浆与子宫输卵管交界处的上皮接触是必不可少的。
